Anti-Cytochrome P450 Reductase 抗体 (ab13513)

製品の概要

  • 製品名Anti-Cytochrome P450 Reductase antibody
    Cytochrome P450 Reductase 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Cytochrome P450 Reductase
  • アプリケーション適用あり: ICC, IP, WB, IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Rat, Sheep, Rabbit, Guinea pig, Hamster, Cow, Dog, Human, Pig, Monkey
  • 免疫原

    Purified rat liver NADPH-Cytochrome P450 reductase.

  • ポジティブ・コントロール
    • Rat Liver NADPH Cytochrome P450 Reductase Protein.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab13513 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC 1/500 - 1/1000.
IP Use a concentration of 5 µg/ml.
WB 1/1000. Detects a band of approximately 78 kDa.
IHC-P 1/500.
ICC/IF Use a concentration of 10 µg/ml.

ターゲット情報

  • 機能This enzyme is required for electron transfer from NADP to cytochrome P450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome B5.
  • 関連疾患Defects in POR are the cause of adrenal hyperplasia variant type (AHV) [MIM:201750]; also known as Antley-Bixler syndrome-like phenotype with disordered steroidogenesis. AHV is a rare variant of congenital adrenal hyperplasia. It is an autosomal recessive disorder with apparent combined P450C17 and P450C21 deficiency. Affected girls are born with ambiguous genitalia, but their circulating androgens are low and virilization does not progress. Conversely, affected boys are sometimes born undermasculinized. Boys and girls can also present with bone malformations, in some cases resembling the pattern seen in patients with Antley-Bixler syndrome.
    Defects in POR are a cause of isolated disordered steroidogenesis (IDS) [MIM:201750].
  • 配列類似性In the C-terminal section; belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.
    Contains 1 FAD-binding FR-type domain.
    Contains 1 flavodoxin-like domain.
  • 細胞内局在Endoplasmic reticulum membrane. Anchored to the ER membrane by its N-terminal hydrophobic region.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CPR antibody
    • CYPOR antibody
    • DKFZp686G04235 antibody
    • FLJ26468 antibody
    • NADPH Cytochrome P450 Reductase antibody
    • NADPH dependent cytochrome P450 reductase antibody
    • NADPH--cytochrome P450 reductase antibody
    • NCPR_HUMAN antibody
    • P450 (cytochrome) oxidoreductase antibody
    • P450 Cytochrome Oxidoreductase antibody
    • P450R antibody
    • por antibody
    see all

Anti-Cytochrome P450 Reductase antibody 画像

  • Anti-Cytochrome P450 Reductase antibody (ab13513) at 1/2000 dilution + Cell lysates prepared from rat PC12 cells
  • ICC/IF image of ab13513 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab13513, 10µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Cytochrome P450 Reductase was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to Cytochrome P450 Reductase and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab13513.
    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
    Band: 77kDa: Cytochrome P450 Reductase

Anti-Cytochrome P450 Reductase antibody (ab13513) 使用論文

This product has been referenced in:
  • Liechtenstein T  et al. A highly efficient tumor-infiltrating MDSC differentiation system for discovery of anti-neoplastic targets, which circumvents the need for tumor establishment in mice. Oncotarget 5:7843-57 (2014). Read more (PubMed: 25151659) »
  • Linnenbaum M  et al. Heme oxygenase isoforms differ in their subcellular trafficking during hypoxia and are differentially modulated by cytochrome P450 reductase. PLoS One 7:e35483 (2012). WB ; Human . Read more (PubMed: 22545110) »

See all 9 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (transfection of GFP tagged Human CYPred)
Loading amount 40000 cells
Specification transfection of GFP tagged Human CYPred
Treatment 1x laemmli buffer, 10 min boiling,
Gel Running Conditions Non-reduced Denaturing (4-20% TGX biorad)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Ms. Anette Orjuela

Verified customer

投稿 Feb 23 2012

Thank you for bringing this to our attention and for sending your protocol and IHC images. I am sorry to see that the antibody is giving you trouble. It is unlikely, but possible, that the wrong antibody was delivered to you. We have not received othe...

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Thank you for contacting us with your question. Ab13513 is provided as whole antiserum and is not a purified antibody. Some of our other cytochrome P450 reductase antibodies are affinity purified (such as ab39995) and we also have purification kits tha...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Liver whole homogenate)
Loading amount 10 µg
Specification Liver whole homogenate
Gel Running Conditions Reduced Denaturing (12 %)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

投稿 Apr 21 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - other (BS2B Human Lung Cells)
Loading amount 30 µg
Specification BS2B Human Lung Cells
Blocking step BSA as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5%
Username

Mr. Adam Szadkowski

Verified customer

投稿 Jun 15 2007

Thank you for contacting us. For ab23677, LTA4 hydrolase, I suggest either a neutrophil or monocyte lysate. We carry THP1 lysate, ab7913, which is a human monocytic leukemia. For a negative control, LTA4 was detected in epidermal keratinocytes and ...

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Thank you for your enquiry. I was able to obtain a Western blot image, which I have added to our datasheet. The antibody was used in WB in this paper, http://cancerres.aacrjournals.org/cgi/content/full/60/13/3550 I hope this information helps. ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"