Anti-Cytochrome P450 3A5 抗体 [EPR4396] (ab108624)

製品の概要

  • 製品名Anti-Cytochrome P450 3A5 antibody [EPR4396]
    Cytochrome P450 3A5 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR4396] to Cytochrome P450 3A5
  • アプリケーション適用あり: WB, IP, IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Cytochrome P450 3A5 aa 250-350.

  • ポジティブ・コントロール
    • WB: Human fetal liver and colon tissue lysates. IHC-P: Human liver and kidney tissues. IP: Human fetal liver tissue lysate.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab108624 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000. Predicted molecular weight: 57 kDa.

For unpurified use at 1/400.

IP 1/50.

For unpurified use at 1/20.

IHC-P 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

See protocols (link: http://www.abcam.com/protocols/ihc-antigen-retrieval-protocol).

For unpurified use at 1/80.

ターゲット情報

  • 機能Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.
  • 配列類似性Belongs to the cytochrome P450 family.
  • 細胞内局在Endoplasmic reticulum membrane. Microsome membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Aryl hydrocarbon hydroxylase antibody
    • CP35 antibody
    • CP3A5_HUMAN antibody
    • CYP3A5 antibody
    • CYPIIIA5 antibody
    • Cytochrome P450 3A5 antibody
    • Cytochrome P450 antibody
    • Cytochrome P450 family 3 subfamily A polypeptide 5 antibody
    • Cytochrome P450 HLp2 antibody
    • Cytochrome P450 subfamily IIIA (niphedipine oxidase) polypeptide 5 antibody
    • Cytochrome P450-PCN3 antibody
    • DKFZp686L16231 antibody
    • Flavoprotein linked monooxygenase antibody
    • FLJ31317 antibody
    • HLp2 antibody
    • Microsomal monooxygenase antibody
    • Niphedipine oxidase antibody
    • P450PCN3 antibody
    • PCN3 antibody
    • Xenobiotic monooxygenase antibody
    see all

Anti-Cytochrome P450 3A5 antibody [EPR4396] 画像

  • Anti-Cytochrome P450 3A5 antibody [EPR4396] (ab108624) at 1/400 dilution (unpurified) + Human fetal liver tissue lysate at 20 µg

    Secondary
    HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size : 57 kDa
    Observed band size : 52 kDa (why is the actual band size different from the predicted?)

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Anti-Cytochrome P450 3A5 antibody [EPR4396] (ab108624) at 1/1000 dilution (purified) + Human fetal liver tissue lysate at 20 µg

    Secondary
    HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size : 57 kDa
    Observed band size : 52 kDa (why is the actual band size different from the predicted?)

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • All lanes : Anti-Cytochrome P450 3A5 antibody [EPR4396] (ab108624) at 1/1000 dilution (unpurified)

    Lane 1 : Human fetal liver tissue lysate
    Lane 2 : Human fetal colon tissue lysate

    Lysates/proteins at 10 µg per lane.


    Predicted band size : 57 kDa
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelling Cytochrome P450 3A5 with unpurified ab108624 at 1/80. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Counterstained with Hematoxylin.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelling Cytochrome P450 3A5 with purified ab108624 at 1/250. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Counterstained with Hematoxylin.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of normal human colon tissue labelling Cytochrome P450 3A5 with unpurified ab108624. Positive staining is shown.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of normal human kidney tissue labelling Cytochrome P450 3A5 with unpurified ab108624. Positive staining is shown.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of normal human breast tissue labelling Cytochrome P450 3A5 with unpurified ab108624. Negative staining is shown.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skeletal muscle tissue labelling Cytochrome P450 3A5 with unpurified ab108624. Negative staining is shown.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of normal human tonsil tissue labelling Cytochrome P450 3A5 with unpurified ab108624. Negative staining is shown.

  • ab108624 (unpurified) at 1/20 immunoprecipitating Cytochrome P450 3A5 in human fetal liver tissue lysate (Lane 1). Lane 2 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • ab108624 (purified) at 1/50 immunoprecipitating Cytochrome P450 3A5 in human fetal liver tissue lysate (Lane 1). Lane 2 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

Anti-Cytochrome P450 3A5 antibody [EPR4396] (ab108624) 使用論文

ab108624 has not yet been referenced specifically in any publications.

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