製品の概要

  • 製品名
    Anti-Cytochrome P450 3A1 antibody
    Cytochrome P450 3A1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Cytochrome P450 3A1
  • アプリケーション
    適用あり: WB, IHC-FoFr, IHC-Frmore details
  • 種交差性
    交差種: Rat
  • 免疫原

    Synthetic peptide conjugated to KLH, taken from the C-terminus of rat Cytochrome P450 3A1.

  • ポジティブ・コントロール
    • PCN stimulated rat liver and microsomal preparations of liver.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab22733 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
IHC-FoFr
IHC-Fr
  • 追加情報
    IHC-F: Use at a dilution of up to 1/2000.
    IHC-Fr: Use at a dilution of up to 1/2000.
    WB: Use at an assay dependent dilution. Detects a band of approximately 58 kDa.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 関連性
      In animals, P-450 enzymes serve two major functions: (1) biosynthesis of steroids, fatty acids, and bile acids; (2) metabolism of endogenous and a wide variety of exogenous substrates, such as toxins and drugs. The four major families involved in drug metabolism are CYP 1,2,3, and 4. Cytochrome P450 3A4 [human-3A1 Rat] is abundantly expressed in liver and small intestine and is inducible by barbiturates, glucocorticoids and rifampicin.
    • 細胞内局在
      Endoplasmic reticulum, membrane bound.
    • 参照データベース
    • 別名
      • CYP3A1 antibody
      • cytochrome P450, family 3, subfamily a, polypeptide 1 antibody

    画像

    • ab22733 staining Cytochrome P450 3A1 in rat liver tissue sections by Immunohistochemistry (frozen sections). Tissue was fixed with acetone. Samples were then blocked with 2% serum for 1 hour at 20°C followed by incubation with the primary antibody, at a 1/500 dilution, for 1 hour at 20°C. An Atto 550 dye-conjugated goat anti-rabbit IgG polyclonal was used as secondary antibody at a 1/500.

      See Abreview

    参考文献

    This product has been referenced in:
    • Dublineau I  et al. Unexpected lack of deleterious effects of uranium on physiological systems following a chronic oral intake in adult rat. Biomed Res Int 2014:181989 (2014). WB ; Rat . Read more (PubMed: 24693537) »
    • Hassan HE  et al. Induction of xenobiotic receptors, transporters, and drug metabolizing enzymes by oxycodone. Drug Metab Dispos 41:1060-9 (2013). Rat . Read more (PubMed: 23439660) »

    See all 3 Publications for this product

    レビューと Q&A

    Thank you for contacting us and I am sorry for the delay in getting back to you.

    I am pleased to hear that the replacement antibody provided has been working well for you. As I mentioned in my previous email, the homology of the CYP3A1 and CYP...

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Rat Tissue sections (Liver)
    Specification
    Liver
    Fixative
    Acetone 10' at 4°C
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 20°C
    Username

    Abcam user community

    Verified customer

    投稿 Oct 12 2010

    I can confirm that the antibodies were raised to a synthetic hexapeptide which includes the C-terminal of rat CYP3A110: H-Cys-Ile-Ile-Thr-Gly-Ser-OH. Both antibodies work equally well in western blotting and we cannot recommend one over the other, w...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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