製品の概要

  • 製品名
    Anti-Cytochrome P450 2E1 antibody
    Cytochrome P450 2E1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Cytochrome P450 2E1
  • 特異性
    The antibody detects a ~50-55 kDa protein, corresponding to the apparent molecular mass of cytochrome P450 IIE1 on SDS-PAGE immunoblots.
  • アプリケーション
    適用あり: IHC-Fr, WB, ICC/IF, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Rat, Rabbit, Horse, Guinea pig, Dog, Human, Monkey
  • 免疫原

    Full length native protein (purified) (Rat)

  • 特記事項


    The antibody has been shown to inhibit microsomal benzene metabolism

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab28146 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr 1/300. PubMed: 17021035
WB 1/5000. Predicted molecular weight: 50-55 kDa. This dilution was sufficient for detection of Cytochrome P450 in 20ug of human liver microsomes by colorimetric immunoblot analysis using Goat anti-Rabbit IgG:AP as the secondary antibody.
ICC/IF 1/1000.
IHC-P Use at an assay dependent concentration. PubMed: 24155903

ターゲット情報

  • 機能
    Metabolizes several precarcinogens, drugs, and solvents to reactive metabolites. Inactivates a number of drugs and xenobiotics and also bioactivates many xenobiotic substrates to their hepatotoxic or carcinogenic forms.
  • 配列類似性
    Belongs to the cytochrome P450 family.
  • 細胞内局在
    Endoplasmic reticulum membrane. Microsome membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 4-nitrophenol 2-hydroxylase antibody
    • CP2E1_HUMAN antibody
    • CPE1 antibody
    • CYP2E antibody
    • Cyp2e1 antibody
    • CYPIIE1 antibody
    • Cytochrome P450 2E1 antibody
    • Cytochrome P450 isozyme 3A antibody
    • Cytochrome P450 LM3A antibody
    • Cytochrome P450, family 2, subfamily e, polypeptide 1 antibody
    • cytochrome P450, subfamily IIE (ethanol-inducible), polypeptide 1 antibody
    • Cytochrome P450, subfamily IIE antibody
    • Cytochrome P450-ALC antibody
    • Cytochrome P450-J antibody
    • Cytochrome P450RLM6 antibody
    • EC 1.14.13.- antibody
    • EC 1.14.13.n7 antibody
    • Ethanol-inducible P450 antibody
    • Flavoprotein-linked monooxygenase antibody
    • MGC133529 antibody
    • Microsomal monooxygenase antibody
    • OTTHUMP00000020813 antibody
    • OTTHUMP00000046571 antibody
    • P450 ALC antibody
    • P450-J antibody
    • P450C2E antibody
    • P450J antibody
    • P450RLM6 antibody
    • Xenobiotic monooxygenase antibody
    see all

画像

  • All lanes : Anti-Cytochrome P450 2E1 antibody (ab28146) at 1/1000 dilution

    Lane 1 : Molecular weight marker
    Lane 2 : Cell lysates prepared from human liver microsomes
    Lane 3 : Cell lysates prepared from rat liver microsomes
    Lane 4 : Cell lysates prepared from mouse liver microsomes
    Lane 5 : Cell lysates prepared from rabbit liver microsomes


    Predicted band size : 50-55 kDa
  • ab28146 staining cells of rat liver sections by IHC-Fr.  Cells were fixed in acetone and blocked with 1% BSA for 5 minutes at 25°C prior to incubating with ab28146 diluted 1/300 for 30 minutes at 25°C.  An Alexa Fluor® 594 conjugated goat anti-rabbit antibody was used as the secondary.

    Double IF labeling with CYP2E1 (Red) and dipeptidyl peptidase IV (Green)

    See Abreview

  • ICC/IF image of ab28146 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28146, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-Cytochrome P450 2E1 antibody (ab28146) at 1/2500 dilution

    Lane 1 : Tissue lysate prepared from normal murine liver
    Lane 2 : Tissue lysate prepared from normal murine liver
    Lane 3 : Tissue lysate prepared from normal murine liver
    Lane 4 : Tissue lysate prepared from normal murine liver

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat anti-rabbit IgG-HRP at 1/5000 dilution
    Developed using the ECL technique

    Predicted band size : 50-55 kDa
    Observed band size : 55 kDa (why is the actual band size different from the predicted?)


    Exposure time : 3 seconds

    Image courtesy of an anonymous Abreview.

    See Abreview

参考文献

This product has been referenced in:
  • Uehara S  et al. Functional characterization and tissue expression of marmoset cytochrome P450 2E1. Biopharm Drug Dispos N/A:N/A (2017). Read more (PubMed: 28474789) »
  • McCracken JM  et al. C57BL/6 Substrains Exhibit Different Responses to Acute Carbon Tetrachloride Exposure: Implications for Work Involving Transgenic Mice. Gene Expr 17:187-205 (2017). Read more (PubMed: 28234577) »

See all 35 Publications for this product

レビューと Q&A

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
capybara Tissue sections (Testis)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate ph 6
Permeabilization
No
Specification
Testis
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 24°C
Fixative
Formaldehyde
Username

Raísa Brito

Verified customer

投稿 Dec 16 2016

Application
Western blot
Sample
Pig Tissue lysate - other (Adipose tissue)
Loading amount
8 µg
Specification
Adipose tissue
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Dec 18 2012

Thank you for kindly confirming these details as this enables us to closely monitor the quality of our products

We would be pleased to arrange a replacement product free of charge of ab28146 from a different lot. I am forwarding these detail...

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Thank you for taking time to answer my questions. I am sorry to hear this antibody is not providing satisfactory results.

The details provided will enable us to investigate this case and will provide us with vital information for monitoring ...

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I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

The details provided will enable us to inve...

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Thank you for taking time to answer my questions.



Having reviewed the answers, I would like to offer some suggestions to help optimize the results from ab28146 Anti-Cytochrome P450 2E1 antibody.



1.I can suggest RIP...

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Thank you for contacting Abcam’s scientific support team.

A replacement is available within 6 months of purchase if the antibody has failed for applications and species listed on the datasheet.

However, with some details of your...

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Thank you for contacting us.

Both mentioned antibodies have been validated to react with rat species. At Abcam, our aim is to share all the information we have available from our products in the Website, to help customers decide the best opti...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - other (Liver)
Loading amount
10 µg
Specification
Liver
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (12% SDS PAGE)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

投稿 Aug 01 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue sections (Liver)
Specification
Liver
Fixative
Acetone 10' at 4°C
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 20°C
Username

Abcam user community

Verified customer

投稿 Oct 12 2010

1-10 of 11 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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