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Rat liver cytochrome P450 2B1 and 2B2.
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The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Predicted molecular weight: 56 kDa.|
|IP||Use a concentration of 5 µg/ml.|
Cytochrome P450 2B1 + 2B2 was immunoprecipitated using 0.5mg Mouse Liver tissue, 5µg of Mouse monoclonal to Cytochrome P450 2B1 + 2B2 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Mouse Liver tissue lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab22719.
Secondary: Goat polyclonal to mouse IgG light chain specific (HRP) at 1/20,000 dilution.
Band: 56kDa; Cytochrome P450 2B1 + 2B2
ab22719 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"