The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/10000. Detects a band of approximately 55 kDa (predicted molecular weight: 57 kDa).
1/10 - 1/100.
1/100 - 1/250.
追加情報Is unsuitable for Flow Cyt or IHC-P.
機能Conversion of pregnenolone and progesterone to their 17-alpha-hydroxylated products and subsequently to dehydroepiandrosterone (DHEA) and androstenedione. Catalyzes both the 17-alpha-hydroxylation and the 17,20-lyase reaction. Involved in sexual development during fetal life and at puberty.
パスウェイLipid metabolism; steroid biosynthesis.
関連疾患Defects in CYP17A1 are the cause of adrenal hyperplasia type 5 (AH5) [MIM:202110]. AH5 is a form of congenital adrenal hyperplasia, a common recessive disease due to defective synthesis of cortisol. Congenital adrenal hyperplasia is characterized by androgen excess leading to ambiguous genitalia in affected females, rapid somatic growth during childhood in both sexes with premature closure of the epiphyses and short adult stature. Four clinical types: "salt wasting" (SW, the most severe type), "simple virilizing" (SV, less severely affected patients), with normal aldosterone biosynthesis, "non-classic form" or late onset (NC or LOAH), and "cryptic" (asymptomatic).
配列類似性Belongs to the cytochrome P450 family.
翻訳後修飾Phosphorylation is necessary for 17,20-lyase, but not for 17-alpha-hydroxylase activity.
Wu S et al. Obesity-induced infertility and hyperandrogenism are corrected by deletion of the insulin receptor in the ovarian theca cell. Diabetes63:1270-82 (2014).
Read more (PubMed: 24379345) »
Dowling AR et al. Genetic factors modulate the impact of pubertal androgen excess on insulin sensitivity and fertility. PLoS One8:e79849 (2013).
Read more (PubMed: 24278193) »