Anti-Cytochrome C Oxidase subunit VIc 抗体 [3G5F7G3] (ab110267)

製品の概要

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab110267 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 5 µg/ml.
Flow Cyt Use 1µg for 106 cells. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
WB Use a concentration of 2 µg/ml. Predicted molecular weight: 9 kDa.

ターゲット情報

  • 機能This protein is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport.
  • 配列類似性Belongs to the cytochrome c oxidase subunit 6c family.
  • 細胞内局在Mitochondrion inner membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • COX 6C antibody
    • Cox6c antibody
    • COX6C_HUMAN antibody
    • Cytochrome c oxidase polypeptide VIc antibody
    • Cytochrome c oxidase subunit 6C antibody
    • cytochrome c oxidase subunit VIc antibody
    • Cytochrome c oxidase subunit VIc preprotein antibody
    see all

Anti-Cytochrome C Oxidase subunit VIc antibody [3G5F7G3] 画像

  • ICC/IF image of ab110267 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab110267, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-Cytochrome C Oxidase subunit VIc antibody [3G5F7G3] (ab110267) at 2 µg/ml

    Lane 1 : Isolated mitochondria from Human heart at 50 µg
    Lane 2 : Isolated mitochondria from Bovine heart at 1 µg
    Lane 3 : Isolated mitochondria from Rat heart at 10 µg


    Predicted band size : 9 kDa
  • Overlay histogram showing HepG2 cells stained with ab110267 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab110267, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-Cytochrome C Oxidase subunit VIc antibody [3G5F7G3] (ab110267) 使用論文

This product has been referenced in:
  • Pérez-Pérez R  et al. COX7A2L Is a Mitochondrial Complex III Binding Protein that Stabilizes the III2+IV Supercomplex without Affecting Respirasome Formation. Cell Rep 16:2387-98 (2016). WB . Read more (PubMed: 27545886) »
  • Sohal RS  et al. Age-related decrease in expression of mitochondrial DNA encoded subunits of cytochrome c oxidase in Drosophila melanogaster. Mech Ageing Dev 129:558-61 (2008). Read more (PubMed: 18538373) »

See all 5 Publications for this product

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