Anti-Cytochrome C Oxidase subunit VIc/COX6C 抗体 [3G5F7G3] (ab110267)
Key features and details
- Mouse monoclonal [3G5F7G3] to Cytochrome C Oxidase subunit VIc/COX6C
- Suitable for: ICC/IF, Flow Cyt, WB
- Reacts with: Rat, Cow, Human
- Isotype: IgG2b
Related conjugates and formulations
製品の概要
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製品名
Anti-Cytochrome C Oxidase subunit VIc/COX6C antibody [3G5F7G3]
Cytochrome C Oxidase subunit VIc/COX6C 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [3G5F7G3] to Cytochrome C Oxidase subunit VIc/COX6C -
由来種
Mouse -
アプリケーション
適用あり: ICC/IF, Flow Cyt, WBmore details -
種交差性
交差種: Rat, Cow, Human -
免疫原
Full length native protein (purified). This information is considered to be commercially sensitive.
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ポジティブ・コントロール
- Isolated mitochondria from Human heart, bovine heart, and rat heart. IF/ICC: HeLa cell line
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特記事項
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
バッファー
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: HEPES buffered saline -
Concentration information loading...
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特記事項(精製)
Near homogeneity as judged by SDS-PAGE. ab110267 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. -
ポリ/モノ
モノクローナル -
クローン名
3G5F7G3 -
アイソタイプ
IgG2b -
軽鎖の種類
kappa -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab110267の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF |
Use a concentration of 5 µg/ml.
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Flow Cyt |
Use 1µg for 106 cells.
ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
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WB |
Use a concentration of 2 µg/ml. Predicted molecular weight: 9 kDa.
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特記事項 |
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ICC/IF
Use a concentration of 5 µg/ml. |
Flow Cyt
Use 1µg for 106 cells. ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
WB
Use a concentration of 2 µg/ml. Predicted molecular weight: 9 kDa. |
ターゲット情報
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機能
This protein is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport. -
配列類似性
Belongs to the cytochrome c oxidase subunit 6c family. -
細胞内局在
Mitochondrion inner membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 1345 Human
- Entrez Gene: 54322 Rat
- Omim: 124090 Human
- SwissProt: P09669 Human
- SwissProt: P11951 Rat
- Unigene: 351875 Human
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別名
- COX 6C antibody
- Cox6c antibody
- COX6C_HUMAN antibody
see all
画像
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All lanes : Anti-Cytochrome C Oxidase subunit VIc/COX6C antibody [3G5F7G3] (ab110267) at 2 µg/ml
Lane 1 : Isolated mitochondria from Human heart at 50 µg
Lane 2 : Isolated mitochondria from Bovine heart at 1 µg
Lane 3 : Isolated mitochondria from Rat heart at 10 µg
Predicted band size: 9 kDa -
ICC/IF image of ab110267 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab110267, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Overlay histogram showing HepG2 cells stained with ab110267 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab110267, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (8)
ab110267 は 8 報の論文で使用されています。
- Pajuelo Reguera D et al. Cytochrome c Oxidase Subunit 4 Isoform Exchange Results in Modulation of Oxygen Affinity. Cells 9:N/A (2020). PubMed: 32075102
- Chang FW et al. Estrogen Enhances the Expression of the Multidrug Transporter Gene ABCG2-Increasing Drug Resistance of Breast Cancer Cells through Estrogen Receptors. Int J Mol Sci 18:N/A (2017). PubMed: 28098816
- Chen X et al. Artesunate Activates the Intrinsic Apoptosis of HCT116 Cells through the Suppression of Fatty Acid Synthesis and the NF-?B Pathway. Molecules 22:N/A (2017). PubMed: 28786914
- Pérez-Pérez R et al. COX7A2L Is a Mitochondrial Complex III Binding Protein that Stabilizes the III2+IV Supercomplex without Affecting Respirasome Formation. Cell Rep 16:2387-98 (2016). WB . PubMed: 27545886
- Sohal RS et al. Age-related decrease in expression of mitochondrial DNA encoded subunits of cytochrome c oxidase in Drosophila melanogaster. Mech Ageing Dev 129:558-61 (2008). PubMed: 18538373
- Janssen GM et al. The A3243G tRNALeu(UUR) mutation induces mitochondrial dysfunction and variable disease expression without dominant negative acting translational defects in complex IV subunits at UUR codons. Hum Mol Genet 16:2472-81 (2007). PubMed: 17656376
- Amaral A et al. The expression of polymerase gamma and mitochondrial transcription factor A and the regulation of mitochondrial DNA content in mature human sperm. Hum Reprod 22:1585-96 (2007). PubMed: 17339235
- Lock RB & Murphy KM Immunodetecting members of the Bcl-2 family of proteins. Methods Mol Med 111:83-96 (2005). PubMed: 15911974