Anti-Cytochrome C Oxidase subunit VIb 抗体 [3F9D3D11AF6] (ab110266)




Our Abpromise guarantee covers the use of ab110266 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 10 kDa.


  • 機能Connects the two COX monomers into the physiological dimeric form.
  • 関連疾患Defects in COX6B1 are a cause of mitochondrial complex IV deficiency (MT-C4D) [MIM:220110]; also known as cytochrome c oxidase deficiency. A disorder of the mitochondrial respiratory chain with heterogeneous clinical manifestations, ranging from isolated myopathy to severe multisystem disease affecting several tissues and organs. Features include hypertrophic cardiomyopathy, hepatomegaly and liver dysfunction, hypotonia, muscle weakness, excercise intolerance, developmental delay, delayed motor development and mental retardation. A subset of patients manifest Leigh syndrome.
  • 配列類似性Belongs to the cytochrome c oxidase subunit 6B family.
  • 細胞内局在Mitochondrion intermembrane space.
  • Information by UniProt
  • 参照データベース
  • 別名
    • COX 6B antibody
    • COX VIb 1 antibody
    • COX VIb-1 antibody
    • COX6B antibody
    • COX6B1 antibody
    • COXG antibody
    • COXVIb1 antibody
    • CX6B1_HUMAN antibody
    • Cytochrome c oxidase subunit 6B1 antibody
    • Cytochrome c oxidase subunit VIb antibody
    • Cytochrome c oxidase subunit VIb isoform 1 antibody
    • cytochrome c oxidase subunit VIb polypeptide 1 (ubiquitous) antibody
    • Cytochrome c oxidase subunit Vib polypeptide 1 antibody
    see all

Anti-Cytochrome C Oxidase subunit VIb antibody [3F9D3D11AF6] 画像

  • ICC/IF image of ab110266 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab110266, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-Cytochrome C Oxidase subunit VIb antibody [3F9D3D11AF6] (ab110266) at 1 µg/ml

    Lane 1 : Isolated mitochondria from Human Heart at 5 µg
    Lane 2 : Isolated mitochondria from Bovine Heart at 4 µg
    Lane 3 : Isolated mitochondria from Rat Heart at 10 µg
    Lane 4 : Isolated mitochondria from Mouse Heart at 10 µg

    Predicted band size : 10 kDa

Anti-Cytochrome C Oxidase subunit VIb antibody [3F9D3D11AF6] (ab110266) 使用論文

This product has been referenced in:
  • Bourens M  et al. Human COX20 cooperates with SCO1 and SCO2 to mature COX2 and promote the assembly of cytochrome c oxidase. Hum Mol Genet N/A:N/A (2014). Human . Read more (PubMed: 24403053) »
  • Lim J  et al. Dual mode action of mangiferin in mouse liver under high fat diet. PLoS One 9:e90137 (2014). WB ; Mouse . Read more (PubMed: 24598864) »

See all 7 Publications for this product

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