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RabMAb

Anti-Cytochrome C 抗体 [EP1326-80-5] (ab76107)

製品の概要

  • 製品名
    Anti-Cytochrome C antibody [EP1326-80-5]
    Cytochrome C 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP1326-80-5] to Cytochrome C
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: WB, IP, IHC-P, ICCmore details
    適用なし: Flow Cyt
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide within Human Cytochrome C (N terminal). The exact sequence is proprietary.

  • ポジティブ・コントロール
    • HeLa, COS and L929 cell lysates; human kidney tissue.
  • 特記事項

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

アプリケーション

Our Abpromise guarantee covers the use of ab76107 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500. Predicted molecular weight: 12 kDa.
IP 1/50.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC 1/50 - 1/100.
  • 追加情報
    Is unsuitable for Flow Cyt.
  • ターゲット情報

    • 機能
      Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain.
      Plays a role in apoptosis. Suppression of the anti-apoptotic members or activation of the pro-apoptotic members of the Bcl-2 family leads to altered mitochondrial membrane permeability resulting in release of cytochrome c into the cytosol. Binding of cytochrome c to Apaf-1 triggers the activation of caspase-9, which then accelerates apoptosis by activating other caspases.
    • 関連疾患
      Defects in CYCS are the cause of thrombocytopenia type 4 (THC4) [MIM:612004]; also known as autosomal dominant thrombocytopenia type 4. Thrombocytopenia is the presence of relatively few platelets in blood. THC4 is a non-syndromic form of thrombocytopenia. Clinical manifestations of thrombocytopenia are absent or mild. THC4 may be caused by dysregulated platelet formation.
    • 配列類似性
      Belongs to the cytochrome c family.
    • 翻訳後修飾
      Binds 1 heme group per subunit.
    • 細胞内局在
      Mitochondrion matrix.
    • Information by UniProt
    • 参照データベース
    • 別名
      • CYC antibody
      • CYC_HUMAN antibody
      • CYCS antibody
      • Cytochrome c antibody
      • Cytochrome c somatic antibody
      • HCS antibody
      • THC4 antibody
      see all

    画像

    • ab76107 at 1/100 dilution staining Cytochrome C in human kidney by Immunohistochemistry using paraffin-embedded tissue.
    • All lanes : Anti-Cytochrome C antibody [EP1326-80-5] (ab76107) at 1/200 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : COS cell lysate
      Lane 3 : L929 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Lanes 1-2 : Goat anti-rabbit HRP at 1/2000 dilution
      Lane 3 : goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 12 kDa
      Observed band size: 15 kDa (why is the actual band size different from the predicted?)

    参考文献

    This product has been referenced in:
    • Liu C  et al. 16-Hydroxycleroda-3, 13-dien-15, 16-olide inhibits the proliferation and induces mitochondrial-dependent apoptosis through Akt, mTOR, and MEK-ERK pathways in human renal carcinoma cells. Phytomedicine 36:95-107 (2017). Read more (PubMed: 29157834) »
    • Xu Z  et al. The protective effect of propofol against TNF-a-induced apoptosis was mediated via inhibiting iNOS/NO production and maintaining intracellular Ca2+homeostasis in mouse hippocampal HT22 cells. Biomed Pharmacother 91:664-672 (2017). Read more (PubMed: 28499237) »

    See all 2 Publications for this product

    レビューと Q&A

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Quercus suber Cell (Secondary Phloem)
    Permeabilization
    No
    Specification
    Secondary Phloem
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
    Fixative
    Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    投稿 Nov 10 2015

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Specification
    HeLa
    Permeabilization
    Yes - 0.5% Triton X100 in PBS
    Fixative
    Paraformaldehyde
    Username

    Dr. Kirk Mcmanus

    Verified customer

    投稿 Apr 04 2014

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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