製品の概要

  • 製品名
    Anti-Cytochrome C antibody
    Cytochrome C 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Cytochrome C
  • 特異性
    ab53056 detects endogenous levels of total Cytochrome C protein.
  • アプリケーション
    適用あり: ELISA, WB, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide derived from human Cytochrome C.

  • ポジティブ・コントロール
    • Extracts from COS7 cells. This antibody gave a positive result when used in the following formaldehyde fixed cell lines: HepG2.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab53056 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/10000.
WB 1/500 - 1/1000. Detects a band of approximately 12 kDa (predicted molecular weight: 12 kDa).
ICC/IF Use a concentration of 10 µg/ml.

ターゲット情報

  • 機能
    Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain.
    Plays a role in apoptosis. Suppression of the anti-apoptotic members or activation of the pro-apoptotic members of the Bcl-2 family leads to altered mitochondrial membrane permeability resulting in release of cytochrome c into the cytosol. Binding of cytochrome c to Apaf-1 triggers the activation of caspase-9, which then accelerates apoptosis by activating other caspases.
  • 関連疾患
    Defects in CYCS are the cause of thrombocytopenia type 4 (THC4) [MIM:612004]; also known as autosomal dominant thrombocytopenia type 4. Thrombocytopenia is the presence of relatively few platelets in blood. THC4 is a non-syndromic form of thrombocytopenia. Clinical manifestations of thrombocytopenia are absent or mild. THC4 may be caused by dysregulated platelet formation.
  • 配列類似性
    Belongs to the cytochrome c family.
  • 翻訳後修飾
    Binds 1 heme group per subunit.
  • 細胞内局在
    Mitochondrion matrix.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CYC antibody
    • CYC_HUMAN antibody
    • CYCS antibody
    • Cytochrome c antibody
    • Cytochrome c somatic antibody
    • HCS antibody
    • THC4 antibody
    see all

Anti-Cytochrome C antibody 画像

  • All lanes : Anti-Cytochrome C antibody (ab53056) at 1/500 dilution

    Lane 1 : extracts from Cos7 cells, no immunising peptide
    Lane 2 : extracts from Cos7 cells, immunising peptide present


    Predicted band size : 12 kDa
    Observed band size : 12 kDa
  • ICC/IF image of ab53056 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab53056 at 10µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Cytochrome C antibody (ab53056) 使用論文

This product has been referenced in:
  • Zhu Y  et al. a- and ß-Naphthoflavone synergistically attenuate H2O2-induced neuron SH-SY5Y cell damage. Exp Ther Med 13:1143-1150 (2017). WB ; Human . Read more (PubMed: 28450955) »
  • Cahova M  et al. Metformin prevents ischemia reperfusion-induced oxidative stress in the fatty liver by attenuation of reactive oxygen species formation. Am J Physiol Gastrointest Liver Physiol 309:G100-11 (2015). Read more (PubMed: 26045616) »

See all 8 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (EL4)
Loading amount
10 µg
Specification
EL4
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 20 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

投稿 Jun 07 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - other (mitochondrial extraction from huh-7 cells)
Loading amount
15 µg
Specification
mitochondrial extraction from huh-7 cells
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 20 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

投稿 Aug 20 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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