Anti-Cytochrome C 抗体 [37BA11] (ab110325)




Our Abpromise guarantee covers the use of ab110325 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.25 - 1 µg/ml. Predicted molecular weight: 12 kDa.
ICC/IF Use a concentration of 0.5 - 2 µg/ml. Heat induced antigen retrieval in 0.1 M Tris-HCl, 5% urea, pH 9.5 for 5 min at 95°C improves signal.
Flow Cyt Use a concentration of 1 µg/ml. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
In-Cell ELISA Use a concentration of 4 µg/ml.


  • 機能Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain.
    Plays a role in apoptosis. Suppression of the anti-apoptotic members or activation of the pro-apoptotic members of the Bcl-2 family leads to altered mitochondrial membrane permeability resulting in release of cytochrome c into the cytosol. Binding of cytochrome c to Apaf-1 triggers the activation of caspase-9, which then accelerates apoptosis by activating other caspases.
  • 関連疾患Defects in CYCS are the cause of thrombocytopenia type 4 (THC4) [MIM:612004]; also known as autosomal dominant thrombocytopenia type 4. Thrombocytopenia is the presence of relatively few platelets in blood. THC4 is a non-syndromic form of thrombocytopenia. Clinical manifestations of thrombocytopenia are absent or mild. THC4 may be caused by dysregulated platelet formation.
  • 配列類似性Belongs to the cytochrome c family.
  • 翻訳後修飾Binds 1 heme group per subunit.
  • 細胞内局在Mitochondrion matrix.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CYC antibody
    • CYC_HUMAN antibody
    • CYCS antibody
    • Cytochrome c antibody
    • Cytochrome c somatic antibody
    • HCS antibody
    • THC4 antibody
    see all

Anti-Cytochrome C antibody [37BA11] 画像

  • ab110325 staining Cytochrome C in MDA MB 231 cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilized with 1% Triton X-100 and blocked with 10% BSA for 1 hour at 21°C. Samples were incubated with primary antibody (1/100) for 1 hour. An undiluted DyLight® 550-conjugated goat anti-mouse IgG polyclonal was used as the secondary antibody.

    See Abreview

  • ab110325 staining cytochrome C in MCF7 cells treated with 15-Deoxy-delta12,14-prostaglandin J2 (ab141717), by ICC/IF. Expression of cytochrome C changes from mitochondrial puncta to a difuse staining pattern with increased concentration of 15-Deoxy-delta12,14-prostaglandin J2, as described in literature.
    The cells were incubated at 37°C for 24 hours in media containing different concentrations of ab141717 (15-Deoxy-delta12,14-prostaglandin J2) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab110325 (10 μg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-mouse polyclonal antibody (ab96879) at 1/250 dilution was used as the secondary antibody. Nuclei (blue) were counterstained with DAPI and membrane is was stained using WGA (red).
  • All lanes : Anti-Cytochrome C antibody [37BA11] (ab110325) at 1 µg/ml

    Lane 1 : Isolated mitochondria from Human heart at 5 µg
    Lane 2 : Isolated mitochondria from Bovine heart at 1 µg
    Lane 3 : Isolated mitochondria from Rat heart at 10 µg
    Lane 4 : Isolated mitochondria from Mouse heart at 10 µg

    Predicted band size : 12 kDa
  • Immunocytochemistry analysis using ab110325 at 2µg/ml staining Cytochrome C in Human fibroblasts (fixed and permeabilized), followed by a fluorescent goat-anti-mouse IgG.
  • Flow cytometric analysis using ab110325 at 1µg/ml staining Cytochrome C in HeLa cells (blue). Isotype control antibody (red).

Anti-Cytochrome C antibody [37BA11] (ab110325) 使用論文

This product has been referenced in:
  • Zhao C  et al. Geniposide Protects Primary Cortical Neurons against Oligomeric Aß1-42-Induced Neurotoxicity through a Mitochondrial Pathway. PLoS One 11:e0152551 (2016). WB ; Mouse . Read more (PubMed: 27046221) »
  • Hwang S  et al. Impaired GAPDH-induced mitophagy contributes to the pathology of Huntington's disease. EMBO Mol Med 7:1307-26 (2015). WB ; Rat . Read more (PubMed: 26268247) »

See all 34 Publications for this product

Product Wall

Application Western blot
Sample Guinea pig Cell lysate - other (Liver, mitochondrial fraction)
Gel Running Conditions Reduced Denaturing (12.5 % acrylamide)
Loading amount 60 µg
Specification Liver, mitochondrial fraction
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 7.5% · Temperature: 22°C

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投稿 May 12 2015

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 21°C
Sample Human Cell (MDA MB 231 cells)
Specification MDA MB 231 cells
Permeabilization Yes - 1% Triton
Fixative Formaldehyde

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投稿 Feb 19 2015

Application Western blot
Loading amount 10 µg
Gel Running Conditions Reduced Denaturing (15)
Sample Human Cell lysate - other (fractionated cells (nuclear, cytosolic, mitochondr)
Specification fractionated cells (nuclear, cytosolic, mitochondr
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 18°C

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Verified customer

投稿 Nov 28 2014

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