製品の概要

  • 製品名Anti-Cysteine Dioxygenase Type 1 antibody
    Cysteine Dioxygenase Type 1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Cysteine Dioxygenase Type 1
  • アプリケーション適用あり: ICC/IF, WB, IP, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Recombinant full length protein (Rat)

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab53436 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 1 µg/ml.
WB 1/2000. Predicted molecular weight: 23 kDa.
IP Use at an assay dependent concentration.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 機能Initiates several important metabolic pathways related to pyruvate and several sulfurate compounds including sulfate, hypotaurine and taurine. Critical regulator of cellular cysteine concentrations. Has an important role in maintaining the hepatic concentation of intracellular free cysteine within a proper narrow range.
  • 組織特異性Highly expressed in liver and placenta. Low expression in heart, brain and pancreas. Also detected in hepatoblastoma HepG2 cells.
  • パスウェイOrganosulfur biosynthesis; taurine biosynthesis; hypotaurine from L-cysteine: step 1/2.
  • 配列類似性Belongs to the cysteine dioxygenase family.
  • 翻訳後修飾The thioether cross-link between Cys-93 and Tyr-157 plays a structural role through stabilizing the Fe(2+) ion, and prevents the production of highly damaging free hydroxyl radicals by holding the oxygen radical via hydroxyl hydrogen.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CDO 1 antibody
    • CDO antibody
    • CDO I antibody
    • CDO-I antibody
    • Cdo1 antibody
    • CDO1_HUMAN antibody
    • CDOI antibody
    • Cysteine dioxygenase type 1 antibody
    • Cysteine dioxygenase type I antibody
    • Cytosolic cysteine dioxygenase antibody
    see all

Anti-Cysteine Dioxygenase Type 1 antibody 画像

  • ICC/IF image of ab53436 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53436, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • IHC image of ab53436 staining in human normal liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab53436, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-Cysteine Dioxygenase Type 1 antibody (ab53436) 使用論文

This product has been referenced in:
  • Prabhu A  et al. Cysteine catabolism: a novel metabolic pathway contributing to glioblastoma growth. Cancer Res 74:787-96 (2014). WB ; Human . Read more (PubMed: 24351290) »
  • Lee SD  et al. TonEBP stimulates multiple cellular pathways for adaptation to hypertonic stress: organic osmolyte-dependent and -independent pathways. Am J Physiol Renal Physiol 300:F707-15 (2011). WB ; Mouse . Read more (PubMed: 21209002) »

See all 2 Publications for this product

Product Wall

Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing (12% gel)
Sample Mouse Tissue lysate - other (liver and kidney)
Specification liver and kidney
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Dr. Hua Jiang

Verified customer

投稿 Jun 17 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Small Cell Lung Cancer)
Loading amount 15 µg
Specification Small Cell Lung Cancer
Treatment CDO1 Overexpressed
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

投稿 Oct 15 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"