製品の概要

  • 製品名Anti-Cystatin C antibody [EPR4413]
    Cystatin C 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR4413] to Cystatin C
  • アプリケーション適用あり: WB, IHC-Pmore details
    適用なし: Flow Cyt,ICC or IP
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Recombinant protein fragment corresponding to residues in Human Cystatin C.

  • ポジティブ・コントロール
    • Human testis, HeLa, HepG2, and U87-MG lysates, Human brain tissue
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab109508 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/10000 - 1/50000. Detects a band of approximately 13 kDa (predicted molecular weight: 15 kDa).
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • 追加情報Is unsuitable for Flow Cyt,ICC or IP.
  • ターゲット情報

    • 機能As an inhibitor of cysteine proteinases, this protein is thought to serve an important physiological role as a local regulator of this enzyme activity.
    • 組織特異性Expressed in submandibular and sublingual saliva but not in parotid saliva (at protein level). Expressed in various body fluids, such as the cerebrospinal fluid and plasma. Expressed in highest levels in the epididymis, vas deferens, brain, thymus, and ovary and the lowest in the submandibular gland.
    • 関連疾患Amyloidosis 6
      Macular degeneration, age-related, 11
    • 配列類似性Belongs to the cystatin family.
    • 翻訳後修飾The Thr-25 variant is O-glycosylated with a core 1 or possibly core 8 glycan. The signal peptide of the O-glycosylated Thr-25 variant is cleaved between Ala-20 and Val-21.
    • 細胞内局在Secreted.
    • Information by UniProt
    • 参照データベース
    • 別名
      • AD 8 antibody
      • AD8 antibody
      • Amyloid angiopathy and cerebral hemorrhage antibody
      • ARMD11 antibody
      • bA218C14.4 (cystatin C) antibody
      • bA218C14.4 antibody
      • Cst 3 antibody
      • Cst3 antibody
      • CST3 protein antibody
      • Cystatin 3 antibody
      • Cystatin-3 antibody
      • Cystatin-C antibody
      • Cystatin3 antibody
      • CystatinC antibody
      • CYTC_HUMAN antibody
      • Gamma trace antibody
      • Gamma-trace antibody
      • HCCAA antibody
      • MGC117328 antibody
      • Neuroendocrine basic polypeptide antibody
      • Post gamma globulin antibody
      • Post-gamma-globulin antibody
      see all

    Anti-Cystatin C antibody [EPR4413] 画像

    • All lanes : Anti-Cystatin C antibody [EPR4413] (ab109508) at 1/10000 dilution

      Lane 1 : Human testis lysate
      Lane 2 : HeLa testis lysate
      Lane 3 : HepG2 testis lysate
      Lane 4 : U87-MG testis lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 15 kDa
      Observed band size : 13 kDa (why is the actual band size different from the predicted?)
    • Immunohistochemical analysis of paraffin-embedded Human brain tissue using ab109508 at adilution of 1/250.

    Anti-Cystatin C antibody [EPR4413] (ab109508) 使用論文

    This product has been referenced in:
    • Wang T  et al. Lycium barbarum polysaccharide prevents focal cerebral ischemic injury by inhibiting neuronal apoptosis in mice. PLoS One 9:e90780 (2014). WB, IHC-P ; Mouse . Read more (PubMed: 24595452) »
    • Spanu S  et al. Urinary exosomes: a novel means to non-invasively assess changes in renal gene and protein expression. PLoS One 9:e109631 (2014). IHC ; Rat . Read more (PubMed: 25310591) »

    See all 2 Publications for this product

    Product Wall

    Application Immunohistochemistry (Frozen sections)
    Sample Mouse Tissue sections (Brain)
    Permeabilization Yes - 0.1 M PBS with 3% Triton X
    Specification Brain
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
    Fixative Paraformaldehyde
    Username

    Dr. Ruma Raha-Chowdhury

    Verified customer

    投稿 Aug 20 2015

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"