Anti-Cystatin C 抗体 (ab85790)


  • 製品名Anti-Cystatin C antibody
    Cystatin C 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Cystatin C
  • アプリケーション適用あり: ICC/IF, WB, IP, Sandwich ELISA, IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Human recombinant Cystatin C.

  • ポジティブ・コントロール
    • HepG2 cell line Human lung FFPE tissue sections




Our Abpromise guarantee covers the use of ab85790 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 1 µg/ml.
WB Use at an assay dependent concentration. Predicted molecular weight: 16 kDa.
IP Use at an assay dependent concentration.
Sandwich ELISA Use a concentration of 0.5 µg/ml. Can be paired for Sandwich ELISA with Mouse monoclonal [Cyst-13] to Cystatin C (ab24327). For sandwich ELISA, use this antibody as Detection at 0.5µg/ml with Ab24327 as Capture.
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.


  • 機能As an inhibitor of cysteine proteinases, this protein is thought to serve an important physiological role as a local regulator of this enzyme activity.
  • 組織特異性Expressed in submandibular and sublingual saliva but not in parotid saliva (at protein level). Expressed in various body fluids, such as the cerebrospinal fluid and plasma. Expressed in highest levels in the epididymis, vas deferens, brain, thymus, and ovary and the lowest in the submandibular gland.
  • 関連疾患Amyloidosis 6
    Macular degeneration, age-related, 11
  • 配列類似性Belongs to the cystatin family.
  • 翻訳後修飾The Thr-25 variant is O-glycosylated with a core 1 or possibly core 8 glycan. The signal peptide of the O-glycosylated Thr-25 variant is cleaved between Ala-20 and Val-21.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • AD 8 antibody
    • AD8 antibody
    • Amyloid angiopathy and cerebral hemorrhage antibody
    • ARMD11 antibody
    • bA218C14.4 (cystatin C) antibody
    • bA218C14.4 antibody
    • Cst 3 antibody
    • Cst3 antibody
    • CST3 protein antibody
    • Cystatin 3 antibody
    • Cystatin-3 antibody
    • Cystatin-C antibody
    • Cystatin3 antibody
    • CystatinC antibody
    • CYTC_HUMAN antibody
    • Gamma trace antibody
    • Gamma-trace antibody
    • HCCAA antibody
    • MGC117328 antibody
    • Neuroendocrine basic polypeptide antibody
    • Post gamma globulin antibody
    • Post-gamma-globulin antibody
    see all

Anti-Cystatin C antibody 画像

  • Standard Curve for Cystatin C (Analyte: ab7653) dilution range 1pg/ml to 1ug/ml using Capture Antibody Mouse monoclonal [Cyst-13] to Cystatin C (ab24327) at 1ug/ml and Detector Antibody Rabbit polyclonal to Cystatin C (ab85790) at 0.5ug/ml
  • ICC/IF image of ab85790 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab85790, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
  • IHC image of Cystatin C staining in human normal lung formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab85790, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.


Anti-Cystatin C antibody (ab85790) 使用論文

ab85790 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (PERIPHERAL BLOOD MONONUCLEAR CELLS)
Gel Running Conditions Reduced Denaturing (15%)
Loading amount 60 µg
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C

Mr. Siddharth Mehra

Verified customer

投稿 Jun 06 2016

Thank you for contacting Abcam.

We originally tested both ab24327 & ab85790 in sandwich ELISA using our Cystain C protein, ab7653 which is unfortunately no longer available in our catalogue.

As an alternative, I would suggest ab87...

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