製品の概要

  • 製品名Anti-Cystatin C antibody
    Cystatin C 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Cystatin C
  • アプリケーション適用あり: IP, ICC/IF, IHC-P, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Rhesus monkey
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Cystatin C.

    (Peptide available as ab68289.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following Human lysates: Caco 2 Whole Cell, U2OS Whole Cell and Spinal Cord Tissue.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab68290 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IP Use a concentration of 5 µg/ml.
ICC/IF Use a concentration of 1 µg/ml.
IHC-P Use a concentration of 1 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 14 kDa (predicted molecular weight: 16 kDa).Can be blocked with Human Cystatin C peptide (ab68289).

ターゲット情報

  • 機能As an inhibitor of cysteine proteinases, this protein is thought to serve an important physiological role as a local regulator of this enzyme activity.
  • 組織特異性Expressed in submandibular and sublingual saliva but not in parotid saliva (at protein level). Expressed in various body fluids, such as the cerebrospinal fluid and plasma. Expressed in highest levels in the epididymis, vas deferens, brain, thymus, and ovary and the lowest in the submandibular gland.
  • 関連疾患Amyloidosis 6
    Macular degeneration, age-related, 11
  • 配列類似性Belongs to the cystatin family.
  • 翻訳後修飾The Thr-25 variant is O-glycosylated with a core 1 or possibly core 8 glycan. The signal peptide of the O-glycosylated Thr-25 variant is cleaved between Ala-20 and Val-21.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • AD 8 antibody
    • AD8 antibody
    • Amyloid angiopathy and cerebral hemorrhage antibody
    • ARMD11 antibody
    • bA218C14.4 (cystatin C) antibody
    • bA218C14.4 antibody
    • Cst 3 antibody
    • Cst3 antibody
    • CST3 protein antibody
    • Cystatin 3 antibody
    • Cystatin-3 antibody
    • Cystatin-C antibody
    • Cystatin3 antibody
    • CystatinC antibody
    • CYTC_HUMAN antibody
    • Gamma trace antibody
    • Gamma-trace antibody
    • HCCAA antibody
    • MGC117328 antibody
    • Neuroendocrine basic polypeptide antibody
    • Post gamma globulin antibody
    • Post-gamma-globulin antibody
    see all

Anti-Cystatin C antibody 画像

  • Cystatin C was immunoprecipitated using 0.5mg Caco2 whole cell extract, 5µg of Rabbit polyclonal to Cystatin C and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).

    The antibody was incubated under agitation with Protein G beads for 10min, Caco2 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.

    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab68290.

    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).

    Band: 14kDa; Cystatin C

  • All lanes : Anti-Cystatin C antibody (ab68290) at 1 µg/ml

    Lane 1 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
    Lane 2 : Human spinal cord tissue lysate - total protein (ab29188)
    Lane 3 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 16 kDa
    Observed band size : 14 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 55 kDa,70 kDa,95 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 10 minutes
  • ICC/IF image of ab68290 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab68290, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) Hek293, HepG2 and MCF7 cells at 1µg/ml, and in 100% methanol fixed (5 min) HeLa, Hek293, HepG2 and MCF7 cells at 1µg/ml.
  • IHC image of Cystatin C staining in Human Liver Cancer FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab68290, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX

  • Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 16 kDa


    Exposure time : 2 minutes

Anti-Cystatin C antibody (ab68290) 使用論文

ab68290 has not yet been referenced specifically in any publications.

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