製品の概要

  • 製品名
    Anti-Cystatin A antibody [WR-23/2/3/3]
    Cystatin A 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [WR-23/2/3/3] to Cystatin A
  • アプリケーション
    適用あり: IHC-P, WBmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Full length protein (Human).

  • ポジティブ・コントロール
    • Human Thymus tissue.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab10442 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/4000. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.
WB Use at an assay dependent concentration. Detects a band of approximately 11-13 kDa.

ターゲット情報

画像

  • IHC image of ab10442 staining in normal human tonsil formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab10442, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

参考文献

This product has been referenced in:
  • Lundberg KC  et al. Proteomics of skin proteins in psoriasis: from discovery and verification in a mouse model to confirmation in humans. Mol Cell Proteomics 14:109-19 (2015). Read more (PubMed: 25351201) »
  • Tang M  et al. Expression and Prognostic Significance of Macrophage Inflammatory Protein-3 Alpha and Cystatin A in Nasopharyngeal Carcinoma. Biomed Res Int 2015:617143 (2015). IHC . Read more (PubMed: 26634210) »

See all 6 Publications for this product

レビューと Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (NIH 3T3 cells)
Loading amount
10 µg
Specification
NIH 3T3 cells
Gel Running Conditions
Reduced Denaturing (4-12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

投稿 Mar 26 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (A431 cells)
Loading amount
10 µg
Specification
A431 cells
Gel Running Conditions
Reduced Denaturing (4-12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

投稿 Mar 26 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録