製品の概要

  • 製品名Anti-CYP7B1 antibody
    CYP7B1 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal to CYP7B1
  • アプリケーション適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant fragment: CDNNKFISEL RDDFLKFDDK FAYLVSNIPI ELLGNVKSIR EKIIKCFSSE KLAKMQGWSE VFQSRQDVLE KYYVHEDLEI GAHH, corresponding to amino acids 203-286 of human CYP7B1 (NP_004811) with a 26 kDa tag.

  • ポジティブ・コントロール
    • CYP7B1 transfected 293T cell lysate. Recombinant tagged human CYP7B1 fragment (the immunogen).

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab77157 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 58 kDa.
ELISA Use at an assay dependent dilution. Detection limit for recombinant tagged CYP7B1 is approximately 0.3 ng/ml when used as a capture antibody.

ターゲット情報

  • 組織特異性Brain, testis, ovary, prostate, liver, colon, kidney, and small intestine.
  • パスウェイLipid metabolism; bile acid biosynthesis.
  • 関連疾患Defects in CYP7B1 are the cause of spastic paraplegia autosomal recessive type 5A (SPG5A) [MIM:270800]. Spastic paraplegia is a neurodegenerative disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs. Rate of progression and the severity of symptoms are quite variable. Initial symptoms may include difficulty with balance, weakness and stiffness in the legs, muscle spasms, and dragging the toes when walking. In some forms of the disorder, bladder symptoms (such as incontinence) may appear, or the weakness and stiffness may spread to other parts of the body.
    Defects in CYP7B1 are the cause of congenital bile acid synthesis defect type 3 (CBAS3) [MIM:613812]. Clinical features include severe cholestasis, cirrhosis and liver synthetic failure. Hepatic microsomal oxysterol 7-alpha-hydroxylase activity is undetectable.
  • 配列類似性Belongs to the cytochrome P450 family.
  • 細胞内局在Endoplasmic reticulum membrane. Microsome membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 25 hydroxycholesterol 7 alpha hydroxylase antibody
    • 25-hydroxycholesterol 7-alpha-hydroxylase antibody
    • CP7B antibody
    • CP7B1_HUMAN antibody
    • Cyp7b1 antibody
    • Cytochrome P450 7B1 antibody
    • Cytochrome P450 family 7 subfamily B polypeptide 1 antibody
    • Cytochrome P450 subfamily VIIB polypeptide 1 antibody
    • Oxysterol 7-alpha-hydroxylase antibody
    • Oxysterol 7alpha hydroxylase antibody
    see all

Anti-CYP7B1 antibody 画像

  • Anti-CYP7B1 antibody (ab77157) at 5 µg/ml + Recombinant tagged human CYP7B1 fragment at 0.2 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

    Predicted band size : 58 kDa
    Observed band size : 35 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-CYP7B1 antibody (ab77157) at 5 µg/ml

    Lane 1 : CYP7B1 transfected 293T cell lysate
    Lane 2 : Non transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 58 kDa
    Observed band size : 58 kDa

Anti-CYP7B1 antibody (ab77157) 使用論文

This product has been referenced in:
  • Zhao YD  et al. De novo synthesize of bile acids in pulmonary arterial hypertension lung. Metabolomics 10:1169-1175 (2014). WB ; Human . Read more (PubMed: 25374487) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"