This fast track antibody is not yet fully characterized. It is subject to these terms and conditions

製品の概要

アプリケーション

Fast track antibodies constitute a diverse group of products that have been released to accelerate your research, but are not yet fully characterized. They have all been affinity purified and show high titre values against the immunizing peptide (by ELISA). Fast track terms of use

アプリケーション Abreviews 特記事項
WB 1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 58 kDa).

ターゲット情報

  • 機能
    Catalyzes a rate-limiting step in cholesterol catabolism and bile acid biosynthesis by introducing a hydrophilic moiety at position 7 of cholesterol. Important for cholesterol homeostasis.
  • 組織特異性
    Detected in liver.
  • パスウェイ
    Lipid metabolism; bile acid biosynthesis.
  • 配列類似性
    Belongs to the cytochrome P450 family.
  • 細胞内局在
    Endoplasmic reticulum membrane. Microsome membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Cholesterol 7 alpha hydroxylase antibody
    • Cholesterol 7 alpha monooxygenase antibody
    • Cholesterol 7-alpha-hydroxylase antibody
    • Cholesterol 7-alpha-monooxygenase antibody
    • CP7A antibody
    • CP7A1_HUMAN antibody
    • CYP 7 antibody
    • CYP7 antibody
    • CYP7A1 antibody
    • CYPVII antibody
    • Cytochrome P450 7A1 antibody
    • Cytochrome P450, family 7, subfamily A, polypeptide 1 antibody
    • Cytochrome P450, subfamily VIIA (cholesterol 7 alpha monooxygenase), antibody
    • Cytochrome P450, subfamily VIIA (cholesterol 7 alpha-monooxygenase), polypeptide 1 antibody
    • MGC126826 antibody
    • MGC138389 antibody
    see all

Anti-CYP7A1 antibody 画像

This Fast-Track antibody is not yet fully characterised. These images represent inconclusive preliminary data.

  • All lanes : Anti-CYP7A1 antibody (ab65596) at 1/1000 dilution

    Lane 1 : Mouse liver lysate
    Lane 2 : Mouse liver lysate

    Lysates/proteins at 30 µg per lane.

    Secondary
    HRP labeled goat anti-rabbit at 1/5000 dilution
    Developed using the ECL technique

    Performed under non-reducing conditions.

    Predicted band size : 58 kDa
    Additional bands at : 40 kDa (possible non-specific binding),70 kDa (possible non-specific binding).

    This image is courtesy of an anonymous Abreview

    Blocking: 5% Milk

    See Abreview

プロトコール

Anti-CYP7A1 antibody (ab65596) 使用論文

This product has been referenced in:
  • Si Y  et al. Phospholipid transfer protein deficiency in mice impairs macrophage reverse cholesterol transport in vivo. Exp Biol Med (Maywood) 241:1466-72 (2016). Read more (PubMed: 27037277) »
  • Zhong CY  et al. Microbiota prevents cholesterol loss from the body by regulating host gene expression in mice. Sci Rep 5:10512 (2015). WB ; Mouse . Read more (PubMed: 26015368) »

See all 8 Publications for this product

Product Wall

Application
Western blot
Sample
Mouse Tissue lysate - whole (liver)
Gel Running Conditions
Non-reduced Denaturing (12%)
Loading amount
30 µg
Specification
liver
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Username

Abcam user community

Verified customer

投稿 Jul 05 2017

Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing (7.5)
Sample
Rat Tissue lysate - whole (liver)
Specification
liver
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 7.5%
Username

Abcam user community

Verified customer

投稿 Sep 09 2013



ab65596 es un llamado “Fast Track” antibody. Se trata de anticuerpos que no se han caracterizado completamente, y que se han añadido al catálogo antes de realizar la validación completa por la necesidad urgent...

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Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hearthe customer hashad difficulty obtaining satisfactory results from this antibody.

The details you have kindly provided will enable us to investigat...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (liver)
Loading amount
30 µg
Specification
liver
Gel Running Conditions
Reduced Denaturing (4-20% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Jan 19 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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