製品の概要

  • 製品名Anti-CYP46 antibody
    CYP46 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to CYP46
  • アプリケーション適用あり: ICC/IF, WB, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Goat, Horse, Guinea pig, Cow, Cat, Dog
  • 免疫原

    synthetic peptide corresponding to a region within internal amino acids 396-445 (YVMGRMDTYF EDPLTFNPDR FGPGAPKPRF TYFPFSLGHR SCIGQQFAQM) of human CYP46 (NP_006659).

  • ポジティブ・コントロール
    • HepG2 Cell lysate This antibody gave a positive result when used in the following formaldehyde fixed cell lines: SKNSH.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab82814 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 1 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 57 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay 1:312500.

ターゲット情報

  • 関連性The Cyp46 gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum protein is expressed exclusively in the brain, where it converts cholesterol to 24S-hydroxycholesterol by adding a hydroxyl group to cholesterol, producing a product that is more soluble than cholesterol and able to be exported from the brain. Cyp46 is also known as 24S-cholesterol hydroxylase
  • 細胞内局在Endoplasmic reticulum
  • 参照データベース
  • 別名
    • Cholesterol 24-hydroxylase antibody
    • CP46 antibody
    • CYP46 antibody
    • cytochrome P450 family 46 antibody

Anti-CYP46 antibody 画像

  • ICC/IF image of ab82814 stained SKNSH cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab82814 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-CYP46 antibody (ab82814) at 1 µg/ml (in 5% skim milk / PBS buffer) + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 57 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 75 kDa. We are unsure as to the identity of these extra bands.

Anti-CYP46 antibody (ab82814) 使用論文

ab82814 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry free floating
Sample Mouse Tissue sections (BRAIN)
Specification BRAIN
Username

Abcam user community

Verified customer

投稿 Sep 21 2016

Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Brain)
Permeabilization Yes - TRITON-X 0.01%
Specification Brain
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Sep 21 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"