製品の概要

  • 製品名Anti-CYP1B1 antibody
    CYP1B1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to CYP1B1
  • アプリケーション適用あり: IHC-P, IHC-Fr, WB, ICC, ICC/IFmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 450 to the C-terminus of Human CYP1B1.

    (Peptide available as ab33585.)

  • ポジティブ・コントロール
    • Human brain tissue lysate.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab33586 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/1500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-Fr Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 61 kDa).Can be blocked with Human CYP1B1 peptide (ab33585).
ICC 1/1000.
ICC/IF 1/500. (see Abreview)

ターゲット情報

  • 機能Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.
    Participates in the metabolism of an as-yet-unknown biologically active molecule that is a participant in eye development.
  • 組織特異性Expressed in many tissues.
  • 関連疾患Defects in CYP1B1 are the cause of primary congenital glaucoma type 3A (GLC3A) [MIM:231300]. GLC3A is an autosomal recessive form of primary congenital glaucoma (PCG). PCG is characterized by marked increase of intraocular pressure at birth or early childhood, large ocular globes (buphthalmos) and corneal edema. It results from developmental defects of the trabecular meshwork and anterior chamber angle of the eye that prevent adequate drainage of aqueous humor.
    Defects in CYP1B1 are a cause of primary open angle glaucoma (POAG) [MIM:137760]. POAG is a complex and genetically heterogeneous ocular disorder characterized by a specific pattern of optic nerve and visual field defects. The angle of the anterior chamber of the eye is open, and usually the intraocular pressure is increased. The disease is asymptomatic until the late stages, by which time significant and irreversible optic nerve damage has already taken place. In some cases, POAG shows digenic inheritance involving mutations in CYP1B1 and MYOC genes.
    Defects in CYP1B1 are a cause of Peters anomaly (PAN) [MIM:604229]. Peters anomaly is a congenital defect of the anterior chamber of the eye.
  • 配列類似性Belongs to the cytochrome P450 family.
  • 細胞内局在Endoplasmic reticulum membrane. Microsome membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Aryl hydrocarbon hydroxylase antibody
    • CP1B antibody
    • CP1B1_HUMAN antibody
    • Cyp1b1 antibody
    • CYPIB1 antibody
    • Cytochrome P450 1B1 antibody
    • Cytochrome P450 family 1 subfamily B polypeptide 1 antibody
    • Cytochrome P450 subfamily I (dioxin inducible) polypeptide 1 (glaucoma 3 primary infantile) antibody
    • Flavoprotein linked monooxygenase antibody
    • GLC3A antibody
    • Microsomal monooxygenase antibody
    • P4501B1 antibody
    • Xenobiotic monooxygenase antibody
    see all

Anti-CYP1B1 antibody 画像

  • Lane 1 : Marker
    Lane 2 : Anti-CYP1B1 antibody (ab33586) at 1 µg/ml

    Lane 1 : As above
    Lane 2 : Human brain tissue lysate at 10 µg

    Secondary
    Lane 2 : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution


    Performed under reducing conditions.

    Predicted band size : 61 kDa
    Observed band size : ~70 kDa (why is the actual band size different from the predicted?)
  • ab33586 staining CYP1B1 in Human AGS Human gastric carcinoma cultured cells by Immunocytochemistry (ICC). Tissue was fixed with formaldehyde, permeabilized with 0.025% Triton-X in PBS and blocked with 5% serum for 1 hour at 23°C. Samples were incubated with primary antibody (1/1000 in blocking buffer) for 16 hours at 4°C. An undiluted HRP-conjugated Goat anti-rabbit polyclonal was used as the secondary antibody.

    See Abreview

Anti-CYP1B1 antibody (ab33586) 使用論文

This product has been referenced in:
  • Rižner TL Estrogen biosynthesis, phase I and phase II metabolism, and action in endometrial cancer. Mol Cell Endocrinol 381:124-39 (2013). Read more (PubMed: 23911898) »
  • Hevir N  et al. Disturbed expression of phase I and phase II estrogen-metabolizing enzymes in endometrial cancer: lower levels of CYP1B1 and increased expression of S-COMT. Mol Cell Endocrinol 331:158-67 (2011). WB, IHC-P ; Human . Read more (PubMed: 20887769) »

See all 3 Publications for this product

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (AGS Human gastric carcinoma cells)
Specification AGS Human gastric carcinoma cells
Fixative Formaldehyde
Permeabilization Yes - 0.025% Triton-X in PBS
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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投稿 Sep 30 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry
Sample Human Cultured Cells (AGS Human gastric carcinoma cells)
Specification AGS Human gastric carcinoma cells
Fixative Formaldehyde
Permeabilization Yes - 0.025% Triton-X in PBS
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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投稿 Sep 30 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Human Tissue sections (Stomach)
Specification Stomach
Fixative Acetone
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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投稿 Jul 19 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Stomach)
Specification Stomach
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10mM citrate pH6.0
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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投稿 Jul 19 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (AGS human gastric carcinoma cells)
Loading amount 20 µg
Specification AGS human gastric carcinoma cells
Gel Running Conditions Reduced Denaturing
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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投稿 Jul 19 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"