製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to CYP11A1
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Cynomolgus monkey
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 400 - 500 of Human CYP11A1.

    (Peptide available as ab95404.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following human lysates: HeLa Whole Cell; HepG2 Whole Cell; Brain Tissue; Testis Tissue; Liver Tissue.

アプリケーション

Our Abpromise guarantee covers the use of ab86357 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa).

ターゲット情報

  • 機能
    Catalyzes the side-chain cleavage reaction of cholesterol to pregnenolone.
  • パスウェイ
    Lipid metabolism; C21-steroid hormone metabolism.
  • 関連疾患
    Defects in CYP11A1 are a cause of congenital adrenal insufficiency (CAI).
    Defects in CYP11A1 are a cause of congenital lipoid adrenal hyperplasia (CLAH) [MIM:201710]; also known as lipoid CAH. CLAH is the most severe form of adrenal hyperplasia. This autosomal recessive and potentially lethal condition includes the onset of profound adrenocortical insufficiency shortly after birth, hyperpigmentation reflecting increased production of pro-opiomelanocortin, elevated plasma renin activity as a consequence of reduced aldosterone synthesis, and male pseudohermaphroditism resulting from deficient fetal testicular testosterone synthesis. CLAH is a rare disease, except in Japan and Korea where it accounts for a significant percentage of cases of congenital adrenal hyperplasia.
  • 配列類似性
    Belongs to the cytochrome P450 family.
  • 細胞内局在
    Mitochondrion membrane.
  • Information by UniProt
  • 参照データベース
    • 別名
      • Cholesterol 20 22 desmolase antibody
      • Cholesterol desmolase antibody
      • Cholesterol monooxygenase (side chain cleaving) antibody
      • Cholesterol side chain cleavage enzyme antibody
      • Cholesterol side chain cleavage enzyme mitochondrial antibody
      • Cholesterol side-chain cleavage enzyme antibody
      • CP11A_HUMAN antibody
      • CYP11A antibody
      • CYP11A1 antibody
      • CYPXIA1 antibody
      • Cytochrome P450 11A1 antibody
      • Cytochrome P450 11A1 mitochondrial antibody
      • Cytochrome P450 family 11 subfamily A polypeptide 1 antibody
      • Cytochrome P450 subfamily XIA antibody
      • Cytochrome P450(scc) antibody
      • Cytochrome P450C11A1 antibody
      • mitochondrial antibody
      • P450SCC antibody
      • Steroid 20 22 lyase antibody
      see all

    画像

    • All lanes : Anti-CYP11A1 antibody (ab86357) at 1 µg/ml

      Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
      Lane 2 : Human brain tissue lysate - total protein (ab29466)
      Lane 3 : Human testis tissue lysate - total protein (ab30257)
      Lane 4 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
      Lane 5 : Human liver tissue lysate - total protein (ab29889)

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) (ab65484) at 1/3000 dilution (Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP))
      Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 60 kDa
      Observed band size : 60 kDa
      Additional bands at : 55 kDa (possible cleavage fragment),80 kDa,85 kDa. We are unsure as to the identity of these extra bands.

      Exposure time : 15 minutes

    プロトコール

    参考文献

    This product has been referenced in:
    • Jia YY  et al. High concentrate-induced subacute ruminal acidosis (SARA) increases plasma acute phase proteins (APPs) and cortisol in goats. Animal 8:1433-8 (2014). Read more (PubMed: 24852750) »

    See 1 Publication for this product

    レビューと Q&A

    Thank you for your enquiry and your interest in our products. I have conducted a search for you and found some antibodies against CYP11A1. I am not sure if the host and the target species is important for your customer. These are all raised agains...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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