CyGEL Sustain™ (ab109205)
製品の概要
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製品名
CyGEL Sustain™ -
アプリケーション
適用あり: ICC/IF, FMmore details -
特記事項
CyGEL Sustain™ is a novel thermoreversible hydrogel which is liquid when cold and a gel when warmed at room temperature (22°C +/- 1°C). CyGEL Sustain™ is compatible with live cells and can be used to immobilize non-adherent cells by simple warming, and conversely, allowing their recovery by simple cooling. CyGEL Sustain™ has many applications in imaging and cell-based screening.
Key features of CyGEL Sustain™ include:- Convenient immobilization of non-adherent cells.
- Controllable and rapid transition from liquid to gel, which allows quick perparation of live cells for gel mounting.
- Optically clear with low autofluorescence.
- Compatible with GFP and other fluorescent probes such as DRAQ5™ (ab108410).
CyGEL Sustain™ is recommended for mounting live de-adherent/ non-adherent cells and Zebrafish embryos for microscopy. CyGEL Sustain™ is specially formulated to allow addition of RPMI and similar culture media, which makes it the best tool for extended imaging experiments, as cells will survive for several hours.
CyGEL Sustain™ is supplied as 10 vials, each containing 500µl.
For live C. elegans and parasites we recommend CyGEL™(ab109204).
製品の特性
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製品の状態
Liquid -
保存方法
Store at +4°C. Do not freeze. -
バッファー
Constituent: 100% Oxirane methyl polymer with oxirane -
Concentration information loading...
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研究分野
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab109205の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF |
Use at an assay dependent concentration.
Please refer to the Protocol Booklet for detailed information about the use of CyGEL Sustain. |
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FM |
Use at an assay dependent concentration.
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特記事項 |
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ICC/IF
Use at an assay dependent concentration. Please refer to the Protocol Booklet for detailed information about the use of CyGEL Sustain. |
FM
Use at an assay dependent concentration. |
画像
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab109205 は 1 報の論文で使用されています。
- Fritzsche M et al. Cytoskeletal actin dynamics shape a ramifying actin network underpinning immunological synapse formation. Sci Adv 3:e1603032 (2017). PubMed: 28691087