CyGEL™ (ab109204)
製品の概要
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製品名
CyGEL™ -
アプリケーション
適用あり: FM, ICC/IFmore details -
特記事項
CyGEL™ is a novel thermoreversible hydrogel which is liquid when cold and a gel when warmed at room temperature (22°C +/- 1°C). CyGEL™ is compatible with live cells and can be used to immobilize non-adherent cells by simple warming, and conversely, allowing their recovery by simple cooling.
CyGEL™ has many applications in imaging and cell-based screening.
Key features of CyGEL™ include:- Convenient immobilization of non-adherent cells.
- Controllable and rapid transition from liquid to gel, which allows quick preparation of live cells for gel mounting.
- Optically clear with low autofluorescence.
- Compatible with GFP and other fluorescent probes such as DRAQ5™ (ab108410).
CyGEL™ is recommended for mounting live de-adherent/ non-adherent cells for microscopy, or mounting live C. elegans and parasites (e.g. leishmania, trypanosomes), which can be recovered afterwards. CyGEL™ is provided as concentrated non-buffered form. Add the concentrated PBS supplement (provided) to prime CyGEL™ prior use.
CyGEL™ is supplied as 8 vials, each containing 500µl, and 1 vial of 40xPBS.
For imaging experiments longer than 2 hours, we recommend using CyGEL Sustain™ (ab109205).
製品の特性
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製品の状態
Liquid -
保存方法
Store at +4°C. Do not freeze. -
バッファー
Constituent: 30% Oxirane methyl polymer with oxirane -
Concentration information loading...
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研究分野
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab109204の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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FM |
Use at an assay dependent concentration.
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ICC/IF |
Use at an assay dependent concentration.
Please refer to the Protocol Booklet for detailed information about the use of CyGEL. |
特記事項 |
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FM
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. Please refer to the Protocol Booklet for detailed information about the use of CyGEL. |
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データシートおよび資料
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Datasheet download
参考文献 (0)
ab109204 は論文での使用が確認できていません。