製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to CYFIP2
  • アプリケーション
    適用あり: WB, IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Zebrafish
  • 免疫原

    Recombinant fragment, corresponding to a region within amino acids 346-610 of Human CYFIP2 (UniProt ID: Q96F07 isoform 2; NP_055191).

  • ポジティブ・コントロール
    • WB: MOLT4 whole cell lysate; Raji cell lysate IHC-P: NCIN87 xenograft

法規制情報

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab95969 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/3000. Predicted molecular weight: 146 kDa.
IHC-P 1/100 - 1/500.
ICC/IF Use at an assay dependent concentration. PubMed: 24658146

ターゲット情報

  • 関連性
    CYFIP2 is involved in T cell adhesion and p53 dependent induction of apoptosis. It does not bind RNA but is up regulated significantly in CD4+ T lymphocytes from patients with multiple sclerosis. There are 2 isoforms produced by alternative splicing.
  • 細胞内局在
    Cytoplasm perinuclear region. Cell junction, synapse, synaptosome. Treatment with leptomycin-B triggers translocation to the nucleus.
  • 参照データベース
  • 別名
    • CYFP2 antibody
    • cytoplasmic FMR1 interacting protein 2 antibody
    • KIAA1168 antibody
    • p53 inducible protein antibody
    • p53-inducible protein 121 antibody
    • PIR121 antibody
    see all

画像

  • Anti-CYFIP2 antibody (ab95969) at 1/1000 dilution + MOLT4 whole cell lysate at 30 µg

    Predicted band size : 146 kDa
  • ab95969, at a 1/100 dilution, staining CYFIP2 in paraffin embedded NCIN87 xenograft by Immunohistochemistry.

参考文献

This product has been referenced in:
  • Han K  et al. Fragile X-like behaviors and abnormal cortical dendritic spines in cytoplasmic FMR1-interacting protein 2-mutant mice. Hum Mol Genet 24:1813-23 (2015). Read more (PubMed: 25432536) »
  • Taha MS  et al. Subcellular fractionation and localization studies reveal a direct interaction of the fragile X mental retardation protein (FMRP) with nucleolin. PLoS One 9:e91465 (2014). ICC/IF ; Human . Read more (PubMed: 24658146) »

See all 4 Publications for this product

レビューと Q&A

Thank you for contacting us. As a general guidance, I can make some suggestions in order to improve the results from this antibody. As a lysis buffer I would suggest using RIPA (radio immuno precipitation assay) buffer. You can prepare it following the...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録