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This antibody recognises Cyclosporin A
Our Abpromise guarantee covers the use of ab8312 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at a dilution of 1/1500 for 2 hrs.
ab8312 staining human PBMC, huh-7 cells by ELISA. Cells were incubated in their respective growth medium with 10% fetal calf serum. At the end of the incubation they were washed with PBS (4°C) and the pellet was used to determine the CsA content.
1st step: cyclophilin B (ab53870, 1/1500 carbonate pH 8.5) was bound to the plate (overnight 4°C), 2nd step blocking agent, 3rd step incubation with cell lysate incubated with CsA or analogs (CsA-OH) overnight,
4th step ab8312 mouse anti CsA, 1/500 for 2 hours at 37°C
5th step ab6729 (1/2000) Alkalline phosphatase reading at 405 nm
ab8312 has not yet been referenced specifically in any publications.