アブカムでは最適な動作のために Google Chrome など最新ブラウザでの閲覧を推奨します。
Recombinant full length protein corresponding to Rat Cyclophilin F aa 1-206. (also known as CypD)
Our Abpromise guarantee covers the use of ab110324 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 22 kDa.|
|Flow Cyt||Use a concentration of 1 µg/ml.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|In-Cell ELISA||Use a concentration of 4 µg/ml.|
|ICC/IF||Use a concentration of 5 µg/ml.|
|IHC-P||1/100. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.|
This image is courtesy of an anonymous Abreview
Blocked with 5% milk for 1 hour at 25°C.
Incubated with the primary antibody diluted in PBS-T + 5% milk for 16 hours at 4°C.
Immunocytochemistry/ Immunofluorescence analysis of HEK293 cells labeling Cyclophilin F with ab110324 at 1/200 dilution. Cells were fixed with paraformaldehyde and permeabilized with 1% triton x-100. 10% goat serum was used to blocke the cells for 1 hour at room temp followed by incubation with Anti-Cyclophilin F antibody [E11AE12BD4] (ab110324) in 10% goat serum-PBST for 16 hours at 4°C. A goat anti-mouse IgG secondary antibody was used at 1/300 dilution.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"