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Recombinant full length protein corresponding to Rat Cyclophilin F aa 1-206. (also known as CypD)
This monoclonal antibody to cyclophilin F has been knockout validated in Western blot. The expected band for cyclophilin F was observed in wild type cells and the band was not seen in knockout cells.
Our Abpromise guarantee covers the use of ab110324 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 22 kDa.|
|Flow Cyt||Use a concentration of 1 µg/ml.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|In-Cell ELISA||Use a concentration of 4 µg/ml.|
|ICC/IF||Use a concentration of 5 µg/ml.|
|IHC-P||1/100. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.|
Lane 1: Wild type HAP1 whole cell lysate (20 µg)
Lane 2: Cyclophilin F knockout HAP1 whole cell lysate (20 µg)
Lane 3: HeLa whole cell lysate (20 µg)
Lane 4: Hek293 whole cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab110324 observed at 24 kDa. Red - loading control, ab181602, observed at 37 kDa.
ab110324 detected the expected band for Cyclophilin F in wild type HAP1 cells and the band was not seen in Cyclophilin F knockout HAP1 cells. Wild-type and Cyclophilin F knockout samples were subjected to SDS-PAGE. Ab110324 and ab181602 (Rabbit anti GAPDH loading control) were incubated overnight at 4°C at 1 ug/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
This image is courtesy of an anonymous Abreview
Blocked with 5% milk for 1 hour at 25°C.
Incubated with the primary antibody diluted in PBS-T + 5% milk for 16 hours at 4°C.
Immunocytochemistry/ Immunofluorescence analysis of HEK293 cells labeling Cyclophilin F with ab110324 at 1/200 dilution. Cells were fixed with paraformaldehyde and permeabilized with 1% triton x-100. 10% goat serum was used to blocke the cells for 1 hour at room temp followed by incubation with Anti-Cyclophilin F antibody [E11AE12BD4] (ab110324) in 10% goat serum-PBST for 16 hours at 4°C. A goat anti-mouse IgG secondary antibody was used at 1/300 dilution.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"