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Our Abpromise guarantee covers the use of ab58144 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 18 kDa.|
|ICC/IF||Use a concentration of 1 µg/ml.|
|ELISA||Use at an assay dependent concentration. It can be applied in indirect ELISA (at a dilution of 1/1500 for 24 hrs).|
|IP||Use at an assay dependent concentration.|
|Flow Cyt||Use 0.5µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.|
This image shows an ELISA analysis of ab58144.
PBMC huh-7 cells were incubated with medium + 10% FCS. At the end of the incubation the supernatant was collected and cells washed with cold PBS. Cells were scrapped and lysate generated. The cell lysate and extracellular medium were assyed for Cyclphilin A content in a sandwich ELISA. The primary antibody was diluted 1/1500 and incubated with the sample for 24 hours at 4°C. Ab6729 was diluted 1/2000.
Key for lower diagram:
1) Rabbit anti-Cyclophilin A (ab3563)
2) Cells samples or Std (ab56523)
3) Mouse anti-Cyclophilin A (ab58144)
4) Rabbit anti-mouse Alkaline Phosphatase (ab6729)
5) Substrate of Alkaline Phosphatase
Standards = Recombinant protein Cyclophilin A (ab56523)