製品の概要

  • 製品名Anti-Cyclophilin A antibody
    Cyclophilin A 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal to Cyclophilin A
  • アプリケーション適用あり: WB, ICC/IF, ELISA, IP, Flow Cytmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant full length protein, corresponding to amino acids 1-166 of Human Cyclophilin A

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab58144 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 18 kDa.
ICC/IF Use a concentration of 1 µg/ml.
ELISA Use at an assay dependent concentration. It can be applied in indirect ELISA (at a dilution of 1/1500 for 24 hrs).
IP Use at an assay dependent concentration.
Flow Cyt Use 0.5µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.
  • 配列類似性Belongs to the cyclophilin-type PPIase family. PPIase A subfamily.
    Contains 1 PPIase cyclophilin-type domain.
  • 細胞内局在Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Cyclophilin A antibody
    • Cyclophilin antibody
    • CyclophilinA antibody
    • Cyclosporin A binding protein antibody
    • Cyclosporin A-binding protein antibody
    • CYPA antibody
    • CYPH antibody
    • Epididymis secretory sperm binding protein Li 69p antibody
    • HEL S 69p antibody
    • MGC117158 antibody
    • MGC12404 antibody
    • MGC23397 antibody
    • Peptidyl prolyl cis trans isomerase A antibody
    • Peptidyl-prolyl cis-trans isomerase A antibody
    • Peptidylprolyl isomerase A (cyclophilin A) antibody
    • Peptidylprolyl isomerase A antibody
    • PPIA antibody
    • PPIA protein antibody
    • PPIA_HUMAN antibody
    • PPIase A antibody
    • Rotamase A antibody
    • RotamaseA antibody
    • T cell cyclophilin antibody
    see all

Anti-Cyclophilin A antibody 画像



  • Predicted band size : 18 kDa
    Cyclophilin A antibody (ab58144) at 1ug/lane + Jurkat cell lysate at 25ug/lane.
  • Cyclophilin A was immunoprecipitated using 0.5mg Hela whole cell extract, 10µg of Mouse monoclonal to Cyclophilin A and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab58144.
    Secondary: Protein G-HRP at 1/500 dilution.
    Band: 18kDa: Cyclophilin A.
  • This image shows an ELISA analysis of ab58144. 

    PBMC huh-7 cells were incubated with medium + 10% FCS.  At the end of the incubation the supernatant was collected and cells washed with cold PBS.  Cells were scrapped and lysate generated.  The cell lysate and extracellular medium were assyed for Cyclphilin A content in a sandwich ELISA.  The primary antibody was diluted 1/1500 and incubated with the sample for 24 hours at 4°C.  Ab6729 was diluted 1/2000.

    Key for lower diagram:

    1) Rabbit anti-Cyclophilin A (ab3563)

    2) Cells samples or Std (ab56523)

    3) Mouse anti-Cyclophilin A (ab58144)

    4) Rabbit anti-mouse Alkaline Phosphatase (ab6729)

    5) Substrate of Alkaline Phosphatase

    Standards = Recombinant protein Cyclophilin A (ab56523)

    See Abreview

  • ICC/IF image of ab58144 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab58144, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Overlay histogram showing HeLa cells stained with ab58144 (red line). The cells were fixed with 4% paraformaldehyde and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab58144, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Anti-Cyclophilin A antibody (ab58144) 使用論文

This product has been referenced in:
  • Saxena R  et al. Proteomic profiling of SupT1 cells reveal modulation of host proteins by HIV-1 Nef variants. PLoS One 10:e0122994 (2015). WB, IF ; Human . Read more (PubMed: 25874870) »
  • Ramachandran S  et al. Proteomic profiling of high glucose primed monocytes identifies cyclophilin A as a potential secretory marker of inflammation in type 2 diabetes. Proteomics 12:2808-21 (2012). Read more (PubMed: 22930659) »

See all 5 Publications for this product

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Tissue lysate - whole (endometrial cancer and adjacent control endometriu)
Loading amount 60 µg
Specification endometrial cancer and adjacent control endometriu
Gel Running Conditions Reduced Denaturing (10 % Tris-Glicine gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

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Verified customer

投稿 Feb 07 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application ELISA
Sample Human Cell (PBMC huh-7 cells)
Specification PBMC huh-7 cells
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 37°C
Type Sandwich (Detection)
Username

Abcam user community

Verified customer

投稿 Jun 30 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"