Anti-Cyclin H/p34 (phospho T315) 抗体 (ab73208)
Key features and details
- Rabbit polyclonal to Cyclin H/p34 (phospho T315)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Cyclin H/p34 (phospho T315) antibody
Cyclin H/p34 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Cyclin H/p34 (phospho T315) -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse -
免疫原
Synthetic peptide corresponding to Human Cyclin H/p34 (phospho T315).
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ポジティブ・コントロール
- HeLa cell extract.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 0.87% Sodium chloride, 50% Glycerol (glycerin, glycerine), PBS
Without Mg2+ and Ca2+ -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
ab73208 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab73208の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/500 - 1/1000. Detects a band of approximately 34 kDa (predicted molecular weight: 38 kDa).
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特記事項 |
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WB
1/500 - 1/1000. Detects a band of approximately 34 kDa (predicted molecular weight: 38 kDa). |
ターゲット情報
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機能
Regulates CDK7, the catalytic subunit of the CDK-activating kinase (CAK) enzymatic complex. CAK activates the cyclin-associated kinases CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive C-terminus domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter and elongation of the transcripts. Involved in cell cycle control and in RNA transcription by RNA polymerase II. Its expression and activity are constant throughout the cell cycle. -
配列類似性
Belongs to the cyclin family. Cyclin C subfamily. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 902 Human
- Entrez Gene: 66671 Mouse
- Omim: 601953 Human
- SwissProt: P51946 Human
- SwissProt: Q61458 Mouse
- Unigene: 292524 Human
- Unigene: 18474 Mouse
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別名
- 6330408H09Rik antibody
- AI661354 antibody
- AV102684 antibody
see all
画像
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All lanes : Anti-Cyclin H/p34 (phospho T315) antibody (ab73208) at 1/500 dilution
Lane 1 : HeLa cell extract
Lane 2 : HeLa cell extract with immunising phosphopeptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 38 kDa
Observed band size: 34 kDa why is the actual band size different from the predicted?
Additional bands at: 90 kDa. We are unsure as to the identity of these extra bands.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab73208 は論文での使用が確認できていません。