Rabbit monoclonal [EPR3929] to Cyclin H
Synthetic peptide within Human Cyclin H aa 300-400. The exact sequence is proprietary.
Jurkat, A431, K562 and HeLa cell lysates, Human breast carcinoma tissue, HeLa cells.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab ® patents
This product is a recombinant rabbit monoclonal antibody.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Tissue culture supernatant
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/10000. Predicted molecular weight: 38 kDa.
1/10 - 1/100.
1/50 - 1/100. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
1/50 - 1/100.
Regulates CDK7, the catalytic subunit of the CDK-activating kinase (CAK) enzymatic complex. CAK activates the cyclin-associated kinases CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive C-terminus domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter and elongation of the transcripts. Involved in cell cycle control and in RNA transcription by RNA polymerase II. Its expression and activity are constant throughout the cell cycle.
Belongs to the cyclin family. Cyclin C subfamily.
Information by UniProt
Western blot - Anti-Cyclin H antibody [EPR3929] (ab92376)
All lanes : Anti-Cyclin H antibody [EPR3929] (ab92376) Lane 1 : Jurkat cell lysate Lane 2 : A431 cell lysate Lane 3 : K562 cell lysate Lane 4 : HeLa cell lysate Lysates/proteins at 10 µg per lane. Secondary All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution Predicted band size: 38 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin H antibody [EPR3929] (ab92376)
ab92376 staining Cyclin H in Human breast carcinoma tissue by Immunohistochemistry Formalin-fixed, Paraffin-embedded tissue. (Detection: DAB staining)
Immunocytochemistry/ Immunofluorescence - Anti-Cyclin H antibody [EPR3929] (ab92376)
ab92376 staining Cyclin H in HeLa cells by Immunofluorescence.
This product has been referenced in:
et al. RelA Ser276 phosphorylation-coupled Lys310 acetylation controls transcriptional elongation of inflammatory cytokines in respiratory syncytial virus infection. J Virol 85:11752-69 (2011).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"