ab54901 at 10 ug/ml staining Cyclin G2 in human Hela cells by Immunocytochemistry / Immunofluorescence.
Western blot - Cyclin G2 antibody (ab54901)This image is courtesy of an anonymous Abreview
All lanes : Anti-Cyclin G2 antibody (ab54901) at 1/1000 dilution (in TBST + 5% milk for 16 hours at 4°C)
Lane 1 : Whole cell lysate of Human U87MG tumour cell line treated with DMSO Lane 2 : Whole cell lysate of Human U87MG tumour cell line treated with 1µM LY294002 for 6 hours Lane 3 : Whole cell lysate of Human U87MG tumour cell line treated with 10µM LY294002 for 6 hours
Lysates/proteins at 50 µg per lane.
Secondary An HRP-conjugated Goat anti-mouse polyclonal at 1/10000 dilution developed using the ECL technique
Hofstetter CP et al. Protein phosphatase 2A mediates dormancy of glioblastoma multiforme-derived tumor stem-like cells during hypoxia. PLoS One7:e30059 (2012).
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