製品の概要

  • 製品名Anti-Cyclin E2 antibody [E142]
    Cyclin E2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [E142] to Cyclin E2
  • 特異性ab32103 recognises Cyclin E2.
  • アプリケーション適用あり: WB, IHC-P, ICC/IF, Flow Cytmore details
    適用なし: IP
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Cyclin E2 aa 350 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: O96020

  • ポジティブ・コントロール
    • Human breast carcinoma, HeLa cells lysates, Jurkat cell lysate
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Alternative versions available:

    Anti-Cyclin E2 antibody (Alexa Fluor® 488) [E142] (ab207336)

    Anti-Cyclin E2 antibody (Alexa Fluor® 647) [E142] (ab207337)

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab32103 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500. Predicted molecular weight: 45 kDa.
IHC-P Use at an assay dependent concentration.
ICC/IF 1/2000.

For unpurified use at 1/100 - 1/250.

Flow Cyt 1/20. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • 追加情報Is unsuitable for IP.
  • ターゲット情報

    • 関連性The human Cyclin E2 gene encodes a 404 amino acid protein that is most closely related to Cyclin E. Cyclin E2 mRNA levels peaks at the G1 / S transition. Cyclin E2 associates with Cdk2 in a functional kinase complex that is inhibited by both p27 (Kip1) and p21 (Cip1). Cyclin E2 / Cdk2 phosphorylates histone H1 in vitro. G1 cyclin E controls the initiation of DNA synthesis by activating CDK2. Abnormally high levels of cyclin E expression have frequently been observed in human cancers. Unlike Cyclin E1, which is expressed in great majority of proliferating normal and neoplastically transformed cells, Cyclin E2 levels are low to undetectable in non transformed cells and increase significantly in neoplasm derived cells.
    • 細胞内局在Nuclear
    • 参照データベース
    • 別名
      • CCN E2 antibody
      • CCNE 2 antibody
      • CCNE2 antibody
      • CCNE2 protein antibody
      • CYC E2 antibody
      • CYCE 2 antibody
      • CYCE2 antibody
      • CyclinE2 antibody
      • G1/S specific cyclin E2 antibody
      see all

    Anti-Cyclin E2 antibody [E142] 画像

    • Anti-Cyclin E2 antibody [E142] (ab32103) at 1/500 dilution + HeLa cell lysate

      Predicted band size : 45 kDa
      Observed band size : 50 kDa (why is the actual band size different from the predicted?)
    • Immunocytochemistry/Immunofluorescence analysis of Jurkat cells labelling Cyclin E2 with purified ab32103 at 1/2000. Cells were fixed with 4% Paraformaldehyde and permeabilised with 0.1% tritonX-100. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (ab150077) at 1/1000 dilution was used as the secondary antibody. Nuclei counterstained with DAPI (blue).

      Secondary Only Control: PBS was used instead of the primary antibody as the negative control.

    • Immunofluorescent analysis of Cyclin E2 expression in HeLa cell culture using 1/100 ab32103.
    • Immunohistochemical analysis of Cyclin E2 expression in paraffin embedded human breast carcinoma using 1/50 ab32103.
    • Overlay histogram showing HeLa cells stained with ab32103 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab32103, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    Anti-Cyclin E2 antibody [E142] (ab32103) 使用論文

    This product has been referenced in:
    • Vogel KU  et al. The RNA-Binding Proteins Zfp36l1 and Zfp36l2 Enforce the Thymic ß-Selection Checkpoint by Limiting DNA Damage Response Signaling and Cell Cycle Progression. J Immunol 197:2673-85 (2016). Read more (PubMed: 27566829) »
    • Suwei D  et al. NLK functions to maintain proliferation and stemness of NSCLC and is a target of metformin. J Hematol Oncol 8:120 (2015). Human . Read more (PubMed: 26503334) »

    See all 6 Publications for this product

    Product Wall

    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.
    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has c...

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    Abcam has not validated the combination of species/application used in this Abreview.
    Application Western blot
    Sample Rat Tissue lysate - whole (Liver)
    Loading amount 50 µg
    Specification Liver
    Gel Running Conditions Reduced Denaturing (12%)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Username

    Abcam user community

    Verified customer

    投稿 Oct 28 2011

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Mouse Cell lysate - whole cell (Hepa 1-6)
    Loading amount 50 µg
    Specification Hepa 1-6
    Gel Running Conditions Reduced Denaturing (12%)
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Username

    Abcam user community

    Verified customer

    投稿 Sep 23 2011

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (HepG2)
    Loading amount 50 µg
    Specification HepG2
    Gel Running Conditions Reduced Denaturing (12)
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Mrs. Sadaf Sultan

    Verified customer

    投稿 Aug 11 2011

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (HeLa cell line)
    Loading amount 50 µg
    Specification HeLa cell line
    Gel Running Conditions Reduced Denaturing
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Username

    Dr. Shakil Ahmad

    Verified customer

    投稿 Aug 11 2011

    Thank you for your enquiry. I can confirm that this product does not require any specific protocols and the general IHC-P and antigen retrieval protocols found on our website (can also be accessed on the datasheet) will be able to help you. From the...

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    Thank you for your enquiry. The approximate concentration of this antibody (ab32103) is 90ug/ml. I hope this is helpful. Please don't hesitate to contact us again if you require more information.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"