製品の概要

  • 製品名Anti-Cyclin E2 antibody
    Cyclin E2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Cyclin E2
  • アプリケーション適用あり: WB, ELISA, IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse
  • 免疫原

    Synthetic non-phosphopeptide derived from human Cyclin E2 around the phosphorylation site of threonine 392 (I-M-TP-P-P).

  • ポジティブ・コントロール
    • Human thyroid gland tissue. Extracts from COLO cells and Jurkat cells. NIH/3T3 cells.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab78941 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Predicted molecular weight: 47 kDa.
ELISA 1/5000.
IHC-P 1/50 - 1/100.
ICC/IF 1/500 - 1/1000.

ターゲット情報

  • 関連性The human Cyclin E2 gene encodes a 404 amino acid protein that is most closely related to Cyclin E. Cyclin E2 mRNA levels peaks at the G1 / S transition. Cyclin E2 associates with Cdk2 in a functional kinase complex that is inhibited by both p27 (Kip1) and p21 (Cip1). Cyclin E2 / Cdk2 phosphorylates histone H1 in vitro. G1 cyclin E controls the initiation of DNA synthesis by activating CDK2. Abnormally high levels of cyclin E expression have frequently been observed in human cancers. Unlike Cyclin E1, which is expressed in great majority of proliferating normal and neoplastically transformed cells, Cyclin E2 levels are low to undetectable in non transformed cells and increase significantly in neoplasm derived cells.
  • 細胞内局在Nuclear
  • 参照データベース
  • 別名
    • CCN E2 antibody
    • CCNE 2 antibody
    • CCNE2 antibody
    • CCNE2 protein antibody
    • CYC E2 antibody
    • CYCE 2 antibody
    • CYCE2 antibody
    • CyclinE2 antibody
    • G1/S specific cyclin E2 antibody
    see all

Anti-Cyclin E2 antibody 画像

  • All lanes : Anti-Cyclin E2 antibody (ab78941) at 1/500 dilution

    Lane 1 : Extracts from COLO cells
    Lane 2 : Extracts from Jurkat cells
    Lane 3 : Extracts from COLO cells with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 47 kDa
    Observed band size : 47 kDa
    Additional bands at : 70 kDa. We are unsure as to the identity of these extra bands.
  • Immunohistochemistry analysis of paraffin-embedded human thyroid gland tissue using ab78941 at 1/50 dilution in the absence (left panel) or presence (right panel) of immunising peptide.
  • Immunofluorescence analysis of NIH/3T3 cells using ab78941 at 1/500 dilution in the absence (left panel) or presence (right panel) of immunising peptide.

Anti-Cyclin E2 antibody (ab78941) 使用論文

ab78941 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"