Anti-Cyclin E1 (phospho T77) 抗体 [EPR2242(2)] (ab124696)

製品の概要

  • 製品名Anti-Cyclin E1 (phospho T77) antibody [EPR2242(2)]
    Cyclin E1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR2242(2)] to Cyclin E1 (phospho T77)
  • 特異性ab124696 only detects Cyclin E1 phosphorylated at Threonine 77.
  • アプリケーション適用あり: WB, IHC-P, ICCmore details
    適用なし: IP
  • 種交差性
    交差種: Human
    非交差種: Mouse, Rat
  • 免疫原

    Synthetic peptide, corresponding to residues surrounding Threonine 77 of Human Cyclin E1.

  • ポジティブ・コントロール
    • JAR cell lysate, treated with Lambda Phosphatase; Human colon tissue.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    A trial size is available to purchase for this antibody.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab124696 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Detects a band of approximately 50 kDa (predicted molecular weight: 47 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC 1/250 - 1/500.
  • 追加情報Is unsuitable for IP.
  • ターゲット情報

    • 機能Essential for the control of the cell cycle at the G1/S (start) transition.
    • 組織特異性Highly expressed in testis and placenta. Low levels in bronchial epithelial cells.
    • 配列類似性Belongs to the cyclin family. Cyclin E subfamily.
    • 翻訳後修飾Phosphorylation of Thr-395 by GSK3 and of Ser-399 by CDK2 accelerates degradation via the ubiquitin proteasome pathway. Phosphorylated upon DNA damage, probably by ATM or ATR.
    • 細胞内局在Nucleus.
    • Information by UniProt
    • 参照データベース
    • 別名
      • CCNE antibody
      • Ccne1 antibody
      • CCNE1_HUMAN antibody
      • cyclin E variant ex5del antibody
      • cyclin E variant ex7del antibody
      • Cyclin E1 antibody
      • Cyclin Es antibody
      • Cyclin Et antibody
      • CyclinE antibody
      • G1/S specific cyclin E antibody
      • G1/S-specific cyclin-E1 antibody
      see all

    Anti-Cyclin E1 (phospho T77) antibody [EPR2242(2)] 画像

    • All lanes : Anti-Cyclin E1 (phospho T77) antibody [EPR2242(2)] (ab124696) at 1/1000 dilution

      Lane 1 : JAR cell lysate, untreated
      Lane 2 : JAR cell lysate, treated with Lambda Phosphatase

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size : 47 kDa
    • ab124696, at 1/100, staining Cyclin E1 (phospho T77) in paraffin-embedded Human colon tissue by Immunohistochemistry.
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    Anti-Cyclin E1 (phospho T77) antibody [EPR2242(2)] (ab124696) 使用論文

    ab124696 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"