製品の概要

  • 製品名Anti-Cyclin E1 antibody [HE12]
    Cyclin E1 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [HE12] to Cyclin E1
  • アプリケーション適用あり: Flow Cyt, ICC/IF, IHC-P, IHC-Fr, WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide corresponding to Human Cyclin E1.

  • ポジティブ・コントロール
    • Breast carcinoma. MCF-7 cells, Hep G2, NIH3T3 cells, breast carcinoma.
  • 特記事項

    This antibody may be diluted to a titer of 1:100 - 1:200 with an enzyme-polymer detection method. The Final dilution sould be determined by the user based upon the staining conditions employed.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab3927 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt 1/10 - 1/50.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ICC/IF 1/50 - 1/200.
IHC-P 1/100 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

This antibody may be diluted to a titer of 1:100 - 1:200 with an enzyme-polymer detection method.

IHC-Fr 1/100 - 1/200.
WB 1/500 - 1/1000. Detects a band of approximately 42 kDa (predicted molecular weight: 47 kDa).

Detects a doublet of 50 kDa and a single band of 42 kDa, see Abreviews.

ターゲット情報

  • 機能Essential for the control of the cell cycle at the G1/S (start) transition.
  • 組織特異性Highly expressed in testis and placenta. Low levels in bronchial epithelial cells.
  • 配列類似性Belongs to the cyclin family. Cyclin E subfamily.
  • 翻訳後修飾Phosphorylation of Thr-395 by GSK3 and of Ser-399 by CDK2 accelerates degradation via the ubiquitin proteasome pathway. Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CCNE antibody
    • Ccne1 antibody
    • CCNE1_HUMAN antibody
    • cyclin E variant ex5del antibody
    • cyclin E variant ex7del antibody
    • Cyclin E1 antibody
    • Cyclin Es antibody
    • Cyclin Et antibody
    • CyclinE antibody
    • G1/S specific cyclin E antibody
    • G1/S-specific cyclin-E1 antibody
    see all

Anti-Cyclin E1 antibody [HE12] 画像

  • Overlay histogram showing MCF-7 cells stained with ab3927 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab3927, 1/10 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1:500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in MCF-7 cells fixed with methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

  • ICC/IF image of ab3927 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3927, 1/100 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) ab150113 used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.



  • Predicted band size : 47 kDa

    Image kindly provided by Tulsiram Prathapam, as part of an abreview.

    Immunoblot analysis of Cyclin E expression in NIH3T3 cells. Cells were transfected with the indicated plasmids and harvested 48 hr post transfection. Cyclin E expression was analysed by immunoblotting with ab3927. Immunoblot analysis of Cyclin E expression in NIH3T3 cells. Cells were transfected with the indicated plasmids and harvested 48 hr post transfection. Cyclin E expression was analysised by immunoblotting with ab3927.

  • ab3927 staining human breast carcinoma by IHC-P.

  • ab3927 - immunohistochemistry

    Formalin fixed paraffin embedded human breast carcinoma stained with Cyclin E, using ABC and DAB chromagen.

Anti-Cyclin E1 antibody [HE12] (ab3927) 使用論文

This product has been referenced in:
  • Bailey ML  et al. Dependence of Human Colorectal Cells Lacking the FBW7 Tumor Suppressor on the Spindle Assembly Checkpoint. Genetics 201:885-95 (2015). WB ; Human . Read more (PubMed: 26354767) »
  • Gonçalves V  et al. Phosphorylation of SRSF1 by SRPK1 regulates alternative splicing of tumor-related Rac1b in colorectal cells. RNA 20:474-82 (2014). Read more (PubMed: 24550521) »

See all 9 Publications for this product

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (HepG2 liver cells)
Gel Running Conditions Reduced Denaturing
Loading amount 50 µg
Specification HepG2 liver cells
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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投稿 Apr 22 2015

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (15%)
Sample Mouse Cell lysate - whole cell (Mouse Pancreatic cancer)
Specification Mouse Pancreatic cancer
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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投稿 Feb 25 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 25°C
Antigen retrieval step Heat mediated
Sample Human Tissue sections (endometiral stromal sarcoma)
Specification endometiral stromal sarcoma
Permeabilization No
Fixative Formaldehyde
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投稿 Jul 30 2013

Thank you for this additional information. One thing to try is using the 1/200 dilution for 1-2 hours room temperature, to see if you can get the target band without getting all the non-specific ones. Please let me know if this does not improve your re...

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I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab3927 with the order number 946601. To check the status of th...

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Something does seem odd about the western blot data. By itself, the slightly slower migration of the cyclin E band is not that concerning. But together with the apparent insensitivity to cyclin E levels, it appears the new vial of ab3927 you received m...

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Thank you for your description of the issues and for including the western blot images. So you are expecting cyclin E to levels to increase due to siEmi1_1?

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HeLA cells)
Loading amount 20 µg
Specification HeLA cells
Gel Running Conditions Reduced Denaturing (10)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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投稿 Aug 25 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HeLa cells)
Loading amount 20 µg
Specification HeLa cells
Treatment 2mM double Thymidine block
Gel Running Conditions Reduced Denaturing (12.5%)
Blocking step Milk as blocking agent for 45 minute(s) · Concentration: 1% · Temperature: 24°C
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投稿 Feb 24 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa cells)
Specification HeLa cells
Fixative Methanol
Permeabilization No
Blocking step BSA as blocking agent for 45 minute(s) · Concentration: 1% · Temperature: 24°C
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投稿 Feb 24 2011

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"