Anti-Cyclin E1 抗体 [HE12] (ab3927)

Anti-Cyclin E1 antibody [HE12] 画像

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  Flow Cytometry - Anti-Cyclin E1 [HE12] antibody (ab3927)

  Overlay histogram showing MCF-7 cells stained with ab3927 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab3927, 1/10 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1:500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in MCF-7 cells fixed with methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.
  
  

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  Immunocytochemistry/ Immunofluorescence - Anti-Cyclin E1 [HE12] antibody (ab3927)

  ICC/IF image of ab3927 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3927, 1/100 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) ab150113 used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
  
  

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  Western blot - Anti-Cyclin E1 [HE12] antibody (ab3927)Image kindly provided by Tulsiram Prathapam, as part of an abreview.
  
  
  Predicted band size : 47 kDa
  

  Image kindly provided by Tulsiram Prathapam, as part of an abreview.

  Immunoblot analysis of Cyclin E expression in NIH3T3 cells. Cells were transfected with the indicated plasmids and harvested 48 hr post transfection. Cyclin E expression was analysed by immunoblotting with ab3927. Immunoblot analysis of Cyclin E expression in NIH3T3 cells. Cells were transfected with the indicated plasmids and harvested 48 hr post transfection. Cyclin E expression was analysised by immunoblotting with ab3927.

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin E1 [HE12] antibody (ab3927)

  ab3927 staining human breast carcinoma by IHC-P.

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin E1 [HE12] antibody (ab3927)

  ab3927 - immunohistochemistry

  Formalin fixed paraffin embedded human breast carcinoma stained with Cyclin E, using ABC and DAB chromagen.