製品の概要
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製品名Anti-Cyclin E1 antibody [HE12]
Cyclin E1 一次抗体 製品一覧 -
製品の詳細Mouse monoclonal [HE12] to Cyclin E1
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由来種Mouse
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アプリケーション適用あり: Flow Cyt, ICC/IF, IHC-P, IHC-Fr, WBmore details
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種交差性交差種: Human
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免疫原
Synthetic peptide corresponding to Human Cyclin E1.
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ポジティブ･コントロール
- Breast carcinoma. MCF-7 cells, Hep G2, NIH3T3 cells, breast carcinoma.
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特記事項
This antibody may be diluted to a titer of 1:100 - 1:200 with an enzyme-polymer detection method. The Final dilution sould be determined by the user based upon the staining conditions employed.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
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バッファーpH: 7.5
Preservative: 0.05% Sodium azide
Constituents: 1% BSA, 99% PBS -
Concentration information loading... -
精製度IgG fraction
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ポリ/モノモノクローナル
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クローン名HE12
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ミエローマunknown
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アイソタイプIgG1
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研究分野
関連製品
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Compatible Secondaries
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Immunohistochemistry kits
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Isotype control
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Positive Controls
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Related Products
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Unmasking reagent
アプリケーション
Our Abpromise guarantee covers the use of ab3927 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt | 1/10 - 1/50. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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| ICC/IF | 1/50 - 1/200. | |
| IHC-P | 1/100 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. This antibody may be diluted to a titer of 1:100 - 1:200 with an enzyme-polymer detection method. |
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| IHC-Fr | 1/100 - 1/200. | |
| WB | 1/500 - 1/1000. Detects a band of approximately 42 kDa (predicted molecular weight: 47 kDa). Detects a doublet of 50 kDa and a single band of 42 kDa, see Abreviews. |
ターゲット情報
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機能Essential for the control of the cell cycle at the G1/S (start) transition.
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組織特異性Highly expressed in testis and placenta. Low levels in bronchial epithelial cells.
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配列類似性Belongs to the cyclin family. Cyclin E subfamily.
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翻訳後修飾Phosphorylation of Thr-395 by GSK3 and of Ser-399 by CDK2 accelerates degradation via the ubiquitin proteasome pathway. Phosphorylated upon DNA damage, probably by ATM or ATR.
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細胞内局在Nucleus.
- Information by UniProt
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参照データベース
- Entrez Gene: 898 Human
- Omim: 123837 Human
- SwissProt: P24864 Human
- Unigene: 244723 Human
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別名
- CCNE antibody
- Ccne1 antibody
- CCNE1_HUMAN antibody
see all
画像
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![Flow Cytometry - Anti-Cyclin E1 antibody [HE12] (ab3927)](http://a.abcam.com/ps/products/3/ab3927/Images/Cyclin-E-Primary-antibodies-ab3927-2.jpg)
Flow Cytometry - Anti-Cyclin E1 antibody [HE12] (ab3927)Overlay histogram showing MCF-7 cells stained with ab3927 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab3927, 1/10 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1:500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in MCF-7 cells fixed with methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.
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![Immunocytochemistry/ Immunofluorescence - Anti-Cyclin E1 antibody [HE12] (ab3927)](http://a.abcam.com/ps/products/3/ab3927/Images/Cyclin-E-Primary-antibodies-ab3927-1.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Cyclin E1 antibody [HE12] (ab3927)ICC/IF image of ab3927 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3927, 1/100 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) ab150113 used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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![Western blot - Anti-Cyclin E1 antibody [HE12] (ab3927)](http://a.abcam.com/ps/products/3/ab3927/Images/ab3927_2.jpg)
Western blot - Anti-Cyclin E1 antibody [HE12] (ab3927)Image kindly provided by Tulsiram Prathapam, as part of an abreview.Immunoblot analysis of Cyclin E expression in NIH/3T3 cells. Cells were transfected with the indicated plasmids and harvested 48 hr post transfection. Cyclin E expression was analysed by immunoblotting with ab3927. Immunoblot analysis of Cyclin E expression in NIH3T3 cells. Cells were transfected with the indicated plasmids and harvested 48 hr post transfection. Cyclin E expression was analysised by immunoblotting with ab3927.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin E1 antibody [HE12] (ab3927)](http://a.abcam.com/ps/products/3/ab3927/Images/ab3927_3.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin E1 antibody [HE12] (ab3927)ab3927 staining human breast carcinoma by IHC-P.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin E1 antibody [HE12] (ab3927)](http://a.abcam.com/ps/products/3/ab3927/Images/ab3927_1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin E1 antibody [HE12] (ab3927)ab3927 - immunohistochemistry
Formalin fixed paraffin embedded human breast carcinoma stained with Cyclin E, using ABC and DAB chromagen.
プロトコール
参考文献
This product has been referenced in:
- Coleman KE et al. SENP8 limits aberrant neddylation of NEDD8 pathway components to promote cullin-RING ubiquitin ligase function. Elife 6:N/A (2017). Read more (PubMed: 28475037) »
- Yu T et al. CBX7 is a glioma prognostic marker and induces G1/S arrest via the silencing of CCNE1. Oncotarget 8:26637-26647 (2017). WB . Read more (PubMed: 28460453) »
レビューと Q&A
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