製品の概要

  • 製品名Anti-Cyclin E1 antibody
    Cyclin E1 一次抗体 製品一覧
  • 製品の詳細
    Mouse polyclonal to Cyclin E1
  • アプリケーション適用あり: WB, ICC/IFmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Full length protein corresponding to Human Cyclin E1.

  • ポジティブ・コントロール
    • Human colon tissue lysate, Cyclin E1 transfected 293T cell line lysate and HeLa cells.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab89585 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 47 kDa.
ICC/IF Use a concentration of 10 µg/ml.

ターゲット情報

  • 機能Essential for the control of the cell cycle at the G1/S (start) transition.
  • 組織特異性Highly expressed in testis and placenta. Low levels in bronchial epithelial cells.
  • 配列類似性Belongs to the cyclin family. Cyclin E subfamily.
  • 翻訳後修飾Phosphorylation of Thr-395 by GSK3 and of Ser-399 by CDK2 accelerates degradation via the ubiquitin proteasome pathway. Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CCNE antibody
    • Ccne1 antibody
    • CCNE1_HUMAN antibody
    • cyclin E variant ex5del antibody
    • cyclin E variant ex7del antibody
    • Cyclin E1 antibody
    • Cyclin Es antibody
    • Cyclin Et antibody
    • CyclinE antibody
    • G1/S specific cyclin E antibody
    • G1/S-specific cyclin-E1 antibody
    see all

Anti-Cyclin E1 antibody 画像

  • Anti-Cyclin E1 antibody (ab89585) at 1 µg/ml + Human colon tissue lysate at 50 µg

    Predicted band size : 47 kDa
    Observed band size : 47 kDa
  • All lanes : Anti-Cyclin E1 antibody (ab89585) at 1 µg/ml

    Lane 1 : Cyclin E transfected 293T cell line lysate
    Lane 2 : Non-transfected 293T cell line lysate

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 47 kDa
    Observed band size : 52 kDa (why is the actual band size different from the predicted?)
  • ab89585 at 10 µg/ml staining Cyclin E in paraformaldehyde fixed, permeabilised HeLa cells by immunofluorescence.

Anti-Cyclin E1 antibody (ab89585) 使用論文

ab89585 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab89585.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"