製品の概要

  • 製品名Anti-Cyclin E1 antibody
    Cyclin E1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Cyclin E1
  • アプリケーション適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Recombinant full length protein corresponding to Human Cyclin E1.

  • ポジティブ・コントロール
    • HepG2 or K562 nuclear lysate Mouse or rat liver nuclear lysate This antibody gave a positive result in IHC in the following FFPE tissue: Human normal cerebral cortex.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab71535 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000. Detects a band of approximately 55 kDa (predicted molecular weight: 47 kDa).
IHC-P 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 機能Essential for the control of the cell cycle at the G1/S (start) transition.
  • 組織特異性Highly expressed in testis and placenta. Low levels in bronchial epithelial cells.
  • 配列類似性Belongs to the cyclin family. Cyclin E subfamily.
  • 翻訳後修飾Phosphorylation of Thr-395 by GSK3 and of Ser-399 by CDK2 accelerates degradation via the ubiquitin proteasome pathway. Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CCNE antibody
    • Ccne1 antibody
    • CCNE1_HUMAN antibody
    • cyclin E variant ex5del antibody
    • cyclin E variant ex7del antibody
    • Cyclin E1 antibody
    • Cyclin Es antibody
    • Cyclin Et antibody
    • CyclinE antibody
    • G1/S specific cyclin E antibody
    • G1/S-specific cyclin-E1 antibody
    see all

Anti-Cyclin E1 antibody 画像

  • IHC image of Cyclin E staining in Human cerebral cortex formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab71535, 1/200 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

     

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • All lanes : Anti-Cyclin E1 antibody (ab71535) at 1/2000 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : K562 cell lysate
    Lane 3 : Mouse liver lysate
    Lane 4 : Rat liver lysate


    Predicted band size : 47 kDa
    Observed band size : 55 kDa (why is the actual band size different from the predicted?)

Anti-Cyclin E1 antibody (ab71535) 使用論文

This product has been referenced in:
  • Serna A  et al. Functional inhibition of aquaporin-3 with a gold-based compound induces blockage of cell proliferation. J Cell Physiol 229:1787-801 (2014). WB ; Rat . Read more (PubMed: 24676973) »

See 1 Publication for this product

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Thank you for contacting Abcam.

The actual predicted molecular weight of Cyclin E is 47kDa, but when we tested it we were detecting bands in the 55kDa region. I do not think that there is anything for you to be concerned about as it could just...

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this h...

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