製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to Cyclin E1
  • 特異性
    Detects endogenous levels of total Cyclin E1 protein.
  • アプリケーション
    適用あり: ELISA, IHC-P, WBmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Synthetic peptide corresponding to Human Cyclin E1.

  • ポジティブ・コントロール
    • Human colon carcinoma tissue, extracts from HeLa cells treated with Paclitasel.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab52189 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/10000.
IHC-P Use at an assay dependent concentration.
WB 1/500 - 1/1000. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).

ターゲット情報

  • 機能
    Essential for the control of the cell cycle at the G1/S (start) transition.
  • 組織特異性
    Highly expressed in testis and placenta. Low levels in bronchial epithelial cells.
  • 配列類似性
    Belongs to the cyclin family. Cyclin E subfamily.
  • 翻訳後修飾
    Phosphorylation of Thr-395 by GSK3 and of Ser-399 by CDK2 accelerates degradation via the ubiquitin proteasome pathway. Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CCNE antibody
    • Ccne1 antibody
    • CCNE1_HUMAN antibody
    • cyclin E variant ex5del antibody
    • cyclin E variant ex7del antibody
    • Cyclin E1 antibody
    • Cyclin Es antibody
    • Cyclin Et antibody
    • CyclinE antibody
    • G1/S specific cyclin E antibody
    • G1/S-specific cyclin-E1 antibody
    see all

画像

  • All lanes : Anti-Cyclin E1 antibody (ab52189) at 1/500 dilution

    Lane 1 : extracts from HeLa cells, treated with Paclitasel (1uM, 60mins)
    Lane 2 : extracts from HeLa cells, treated with Paclitasel (1uM, 60mins) with blocking peptide


    Predicted band size : 47 kDa
    Observed band size : 47 kDa
  • Ab52189 at 1/50 dilution staining Human colon carcinoma, with and without blocking peptide; paraffin embedded.Ab52189 at 1/50 dilution staining Human colon carcinoma, with and without blocking peptide; paraffin embedded.

参考文献

This product has been referenced in:
  • Chao C  et al. Epstein-Barr virus infection and expression of B-cell oncogenic markers in HIV-related diffuse large B-cell Lymphoma. Clin Cancer Res 18:4702-12 (2012). Read more (PubMed: 22711707) »
  • Ramathal C  et al. Lack of CCAAT enhancer binding protein beta (C/EBPbeta) in uterine epithelial cells impairs estrogen-induced DNA replication, induces DNA damage response pathways, and promotes apoptosis. Mol Cell Biol 30:1607-19 (2010). IHC-P ; Mouse . Read more (PubMed: 20086097) »

See all 3 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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