Anti-Cyclin D3/CCND3 (phospho T283) 抗体 (ab55322)
Key features and details
- Rabbit polyclonal to Cyclin D3/CCND3 (phospho T283)
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Cyclin D3/CCND3 (phospho T283) antibody
Cyclin D3/CCND3 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Cyclin D3/CCND3 (phospho T283) -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat -
免疫原
Synthetic peptide corresponding to Human Cyclin D3/CCND3 aa 200-300 (phospho T283).
Database link: P30281 -
ポジティブ・コントロール
- Human lung carcinoma tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab55322の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
Use at an assay dependent concentration.
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WB |
1/500 - 1/1000.
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特記事項 |
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IHC-P
Use at an assay dependent concentration. |
WB
1/500 - 1/1000. |
ターゲット情報
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機能
Regulatory component of the cyclin D3-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D3/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex. -
配列類似性
Belongs to the cyclin family. Cyclin D subfamily.
Contains 1 cyclin N-terminal domain. -
細胞内局在
Nucleus. Cytoplasm. Membrane. Cyclin D-CDK4 complexes accumulate at the nuclear membrane and are then translocated to the nucleus through interaction with KIP/CIP family members. - Information by UniProt
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参照データベース
- Entrez Gene: 896 Human
- Entrez Gene: 12445 Mouse
- Entrez Gene: 25193 Rat
- Omim: 123834 Human
- SwissProt: P30281 Human
- SwissProt: P30282 Mouse
- SwissProt: P48961 Rat
- Unigene: 534307 Human
see all -
別名
- CCND 3 antibody
- Ccnd3 antibody
- CCND3_HUMAN antibody
see all
画像
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Anti-Cyclin D3/CCND3 (phospho T283) antibody (ab55322) + K562 cell lysate (treated with UV) blocked with phospho peptide.
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This image shows human lung carcinoma tissue stained with ab55322 at 1/50 dilution. The left hand image shows untreated tissue, the right hand image shows tissue treated with the immunising phosphopeptide.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab55322 は 2 報の論文で使用されています。
- Fruit C et al. Biological Characterization of 8-Cyclopropyl-2-(pyridin-3-yl)thiazolo[5,4-f]quinazolin-9(8H)-one, a Promising Inhibitor of DYRK1A. Pharmaceuticals (Basel) 12:N/A (2019). PubMed: 31861110
- Jones K et al. GSK3ß mediates muscle pathology in myotonic dystrophy. J Clin Invest 122:4461-72 (2012). WB ; Human . PubMed: 23160194