製品の概要

  • 製品名Anti-Cyclin D3 (phospho T283) antibody
    Cyclin D3 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Cyclin D3 (phospho T283)
  • 特異性This antibody detects endogenous levels of Cyclin D3 only when phosphorylated at threonine 283.
  • アプリケーション適用あり: ELISA, IHC-P, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat
  • 免疫原

    Synthetic phosphopeptide derived from human Cyclin D3 around the phosphorylation site of threonine 283 (T-S-TP-P-T).

  • ポジティブ・コントロール
    • Human lung carcinoma tissue.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab55322 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/5000.
IHC-P Use at an assay dependent concentration.
WB Use at an assay dependent concentration. PubMed: 23160194

ターゲット情報

  • 機能Regulatory component of the cyclin D3-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D3/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex.
  • 配列類似性Belongs to the cyclin family. Cyclin D subfamily.
    Contains 1 cyclin N-terminal domain.
  • 細胞内局在Nucleus. Cytoplasm. Membrane. Cyclin D-CDK4 complexes accumulate at the nuclear membrane and are then translocated to the nucleus through interaction with KIP/CIP family members.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CCND 3 antibody
    • Ccnd3 antibody
    • CCND3_HUMAN antibody
    • CyclinD3 antibody
    • D3 type cyclin antibody
    • G1 S specific cyclin D3 antibody
    • G1/S specific cyclin D3 antibody
    • G1/S-specific cyclin-D3 antibody
    see all

Anti-Cyclin D3 (phospho T283) antibody 画像

  • This image shows human lung carcinoma tissue stained with ab55322 at 1/50 dilution. The left hand image shows untreated tissue, the right hand image shows tissue treated with the immunising phosphopeptide.

Anti-Cyclin D3 (phospho T283) antibody (ab55322) 使用論文

This product has been referenced in:

See 1 Publication for this product

Product Wall

Application Western blot
Loading amount 10 µg
Gel Running Conditions Reduced Denaturing
Sample Mouse Cell lysate - whole cell (pre-B cells)
Specification pre-B cells
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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投稿 Nov 20 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"