The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 33 kDa.
Regulatory component of the cyclin D2-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D2/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex.
Belongs to the cyclin family. Cyclin D subfamily. Contains 1 cyclin N-terminal domain.
Nucleus. Cytoplasm. Membrane. Cyclin D-CDK4 complexes accumulate at the nuclear membrane and are then translocated into the nucleus through interaction with KIP/CIP family members.
All lanes : Anti-Cyclin D2 antibody (ab81359) at 1 µg/ml
Lane 1 : Tissue lysates prepared from Rat testicle tissue. Lane 2 : Tissue lysates prepared from Rat ovary tissue. Lane 3 : Tissue lysates prepared from Rat placental tissue. Lane 4 : Whole cell lysates prepared from MCF7 cells. Lane 5 : Whole cell lysates prepared from MM231 cells. Lane 6 : Whole cell lysates prepared from Hela cells. Lane 7 : Whole cell lysates prepared from HT1080 cells. Lane 8 : Whole cell lysates prepared from colo320 cells.
Lysates/proteins at 50 µg per lane.
Secondary HRP-conjugated Goat anti-rabbit IgG at 1/3000 dilution