製品の概要

  • 製品名Anti-Cyclin D1 antibody [CD1.1]
    Cyclin D1 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [CD1.1] to Cyclin D1
  • 特異性The antibody CD1.1 recognizes cyclin D1, an ubiquitously expressed 33 kDa protein that migrates as a 36 kDa band under reducing SDS-PAGE conditions.
  • アプリケーション適用あり: IHC-P, IHC-Fr, Flow Cyt, WB, IP, ELISA, ICCmore details
  • 種交差性
    交差種: Mouse, Rat, Human
    交差が予測される動物種: Chicken, Cow, Dog, Orangutan
  • 免疫原

    Full length protein corresponding to Cyclin D1. Purified cyclin D1 protein.
    Sequence:

    MEHQLLCCEVETIRRAYPDANLLNDRVLRAMLKAEETCAPSVSYFKCVQK EVLPSMRKIV ATWMLEVCEEQKCEEEVFPLAMNYLDRFLSLEPVKKSR LQLLGATCMFVASKMKETIPLT AEKLCIYTDNSIRPEELLQMELLLVN KLKWNLAAMTPHDFIEHFLSKMPEAEENKQIIRK HAQTFVALCATDVK FISNPPSMVAAGSVVAAVQGLNLRSPNNFLSYYRLTRFLSRVIKCD PD CLRACQEQIEALLESSLRQAQQNMDPKAAEEEEEEEEEVDLACTPTDVRD VDI


    Database link: P24385

  • ポジティブ・コントロール
    • Small intestine, colon

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab6152 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/1000 - 1/10000.

We recommend antigen retrieval with sodium citrate buffer (10mM Sodium Citrate, 0.05% Tween 20, pH 6.0) before IHC staining.

IHC-Fr Use a concentration of 2 µg/ml.
Flow Cyt Use at an assay dependent concentration.

Membrane permeabilization is required.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

WB 1/500. Detects a band of approximately 36 kDa (predicted molecular weight: 33 kDa).
IP Use a concentration of 1 µg/ml.
ELISA Use at an assay dependent concentration.
ICC Use a concentration of 1 µg/ml.

ターゲット情報

  • 機能Essential for the control of the cell cycle at the G1/S (start) transition.
  • 関連疾患Note=A chromosomal aberration involving CCND1 may be a cause of B-lymphocytic malignancy, particularly mantle-cell lymphoma (MCL). Translocation t(11;14)(q13;q32) with immunoglobulin gene regions. Activation of CCND1 may be oncogenic by directly altering progression through the cell cycle.
    Note=A chromosomal aberration involving CCND1 may be a cause of parathyroid adenomas. Translocation t(11;11)(q13;p15) with the parathyroid hormone (PTH) enhancer.
    Defects in CCND1 are a cause of multiple myeloma (MM) [MIM:254500]. MM is a malignant tumor of plasma cells usually arising in the bone marrow and characterized by diffuse involvement of the skeletal system, hyperglobulinemia, Bence-Jones proteinuria and anemia. Complications of multiple myeloma are bone pain, hypercalcemia, renal failure and spinal cord compression. The aberrant antibodies that are produced lead to impaired humoral immunity and patients have a high prevalence of infection. Amyloidosis may develop in some patients. Multiple myeloma is part of a spectrum of diseases ranging from monoclonal gammopathy of unknown significance (MGUS) to plasma cell leukemia. Note=A chromosomal aberration involving CCND1 is found in multiple myeloma. Translocation t(11;14)(q13;q32) with the IgH locus.
  • 配列類似性Belongs to the cyclin family. Cyclin D subfamily.
  • 翻訳後修飾Phosphorylation at Thr-286 by MAP kinases is required for ubiquitination and degradation following DNA damage. It probably plays an essential role for recognition by the FBXO31 component of SCF (SKP1-cullin-F-box) protein ligase complex.
    Ubiquitinated, primarily as 'Lys-48'-linked polyubiquitination. Ubiquitinated by a SCF (SKP1-CUL1-F-box protein) ubiquitin-protein ligase complex containing FBXO4 and CRYAB (By similarity). Following DNA damage it is ubiquitinated by some SCF (SKP1-cullin-F-box) protein ligase complex containing FBXO31. Ubiquitination leads to its degradation and G1 arrest. Deubiquitinated by USP2; leading to stabilize it.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • AI327039 antibody
    • B cell CLL/lymphoma 1 antibody
    • B cell leukemia 1 antibody
    • B cell lymphoma 1 protein antibody
    • B-cell lymphoma 1 protein antibody
    • BCL 1 antibody
    • BCL-1 antibody
    • BCL-1 oncogene antibody
    • BCL1 antibody
    • BCL1 oncogene antibody
    • ccnd1 antibody
    • CCND1/FSTL3 fusion gene, included antibody
    • CCND1/IGHG1 fusion gene, included antibody
    • CCND1/IGLC1 fusion gene, included antibody
    • CCND1/PTH fusion gene, included antibody
    • CCND1_HUMAN antibody
    • cD1 antibody
    • Cyl 1 antibody
    • D11S287E antibody
    • G1/S specific cyclin D1 antibody
    • G1/S-specific cyclin-D1 antibody
    • Parathyroid adenomatosis 1 antibody
    • PRAD1 antibody
    • PRAD1 oncogene antibody
    • U21B31 antibody
    see all

Anti-Cyclin D1 antibody [CD1.1] 画像

  • Immunohistochemical analysis of Huh7 tumour xenografts grown in mice treated with either control (left) or anti-Wnt1 antibody (right), staining Cyclin D1 with ab6152. Sections were fixed in acetone before incubation with primary antibody (1/250). Staining was detected using HRP.

Anti-Cyclin D1 antibody [CD1.1] (ab6152) 使用論文

This product has been referenced in:
  • Chen Z  et al. Suppressive effect of formononetin on platelet-derived growth factor-BB-stimulated proliferation and migration of vascular smooth muscle cells. Exp Ther Med 12:1901-1907 (2016). Human . Read more (PubMed: 27588108) »
  • Suwei D  et al. NLK functions to maintain proliferation and stemness of NSCLC and is a target of metformin. J Hematol Oncol 8:120 (2015). Human . Read more (PubMed: 26503334) »

See all 18 Publications for this product

Product Wall

Application Western blot
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing (4-12%)
Sample Human Cell lysate - whole cell (HepG2)
Specification HepG2
Treatment 0.1mM Iron for 24hrs
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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投稿 Oct 27 2014

Application Western blot
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing (4-12%)
Sample Rat Tissue lysate - whole (liver)
Specification liver
Treatment TO for 48hrs
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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投稿 Oct 27 2014

Application Western blot
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing (4-12%)
Sample Mouse Tissue lysate - whole (liver)
Specification liver
Treatment TO for 36hrs
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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投稿 Oct 27 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Human Fibroblast)
Loading amount 15 µg
Specification Human Fibroblast
Gel Running Conditions Reduced Denaturing (4-12% Bis-Tris Nupage gel)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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投稿 Feb 25 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (glioblastoma cells)
Loading amount 50 µg
Specification glioblastoma cells
Gel Running Conditions Non-reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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投稿 Dec 04 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Rat Tissue sections (Liver)
Specification Liver
Fixative Acetone
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 21°C
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投稿 Nov 29 2012

Thank you for confirming this information and for your help and cooperation with this case.

As requested, I have asked our accounting department to issue you with a credit note. This can then be redeemed against the invoice of a future order...

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I am sorry to hear the suggestions have not improved the results on this occasion. I fully understand the concerns of the customerand it is disappointing the results have not been successful.

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Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear the customer has had difficulty obtaining satisfactory results from this antibody.

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Thank you for your reply.

All three of these antibodies were validated using a protocol similar to the following:

http://www.abcam.com/index.html?pageconfig=resource&rid=11389

These antibodies were tested using BSA as a...

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