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Full length protein corresponding to Cyclin D1. Purified cyclin D1 protein.
MEHQLLCCEVETIRRAYPDANLLNDRVLRAMLKAEETCAPSVSYFKCVQK EVLPSMRKIV ATWMLEVCEEQKCEEEVFPLAMNYLDRFLSLEPVKKSR LQLLGATCMFVASKMKETIPLT AEKLCIYTDNSIRPEELLQMELLLVN KLKWNLAAMTPHDFIEHFLSKMPEAEENKQIIRK HAQTFVALCATDVK FISNPPSMVAAGSVVAAVQGLNLRSPNNFLSYYRLTRFLSRVIKCD PD CLRACQEQIEALLESSLRQAQQNMDPKAAEEEEEEEEEVDLACTPTDVRD VDI
Our Abpromise guarantee covers the use of ab6152 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/1000 - 1/10000.
We recommend antigen retrieval with sodium citrate buffer (10mM Sodium Citrate, 0.05% Tween 20, pH 6.0) before IHC staining.
|IHC-Fr||Use a concentration of 2 µg/ml.|
|Flow Cyt||Use at an assay dependent concentration.
Membrane permeabilization is required.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|WB||1/500. Detects a band of approximately 36 kDa (predicted molecular weight: 33 kDa).|
|IP||Use a concentration of 1 µg/ml.|
|ELISA||Use at an assay dependent concentration.|
|ICC||Use a concentration of 1 µg/ml.|
Flow Cytometry analysis of human Breast cancer (MDA-MB-436) cells labeling Cyclin D1 with ab6152 at 1/50 dilution. Sub-confluent cells were harvested via trypsinisation with trypsin/EDTA in 1x PBS with 5% FCS. Live (non apoptotic cells) were selected as the gating strategy. A polyclonal horse anti-mouse IgG FITC conjugated secondary antibody was used at 1/200 dilution.
Immunohistochemistry (Frozen sections) analysis of rat liver tissue sections labeling Cyclin D1 with ab6152 at 1/200 dilution. Tissue sections were fixed with acetone and blocked with 10% serum for 1 hour at 21°C. A polyclonal Goat anti-mouse Alexa Fluor® 594 was used as the secondary antibody at 1/1000 dilution.
This image is courtesy of an anonymous abreview.