製品の概要

  • 製品名
    Anti-Cyclin B1 (phospho S147) antibody
    Cyclin B1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Cyclin B1 (phospho S147)
  • アプリケーション
    適用あり: ELISA, IHC-P, WBmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Synthetic peptide corresponding to Human Cyclin B1 (phospho S147). Immunogen range is aa 121-170.
    Database link: P14635

  • ポジティブ・コントロール
    • Human placenta tissue, Jurkat cell extracts treated with UV light.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab60986 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/10000.
IHC-P 1/50 - 1/100.
WB 1/500 - 1/1000. Detects a band of approximately >48 kDa (predicted molecular weight: 48 kDa).

ターゲット情報

  • 機能
    Essential for the control of the cell cycle at the G2/M (mitosis) transition.
  • 配列類似性
    Belongs to the cyclin family. Cyclin AB subfamily.
  • 発生段階
    Accumulates steadily during G2 and is abruptly destroyed at mitosis.
  • 翻訳後修飾
    Ubiquitinated by the SCF(NIPA) complex during interphase, leading to its destruction. Not ubiquitinated during G2/M phases.
  • 細胞内局在
    Cytoplasm. Nucleus. Cytoplasm > cytoskeleton > centrosome.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CCNB 1 antibody
    • CCNB antibody
    • ccnb1 antibody
    • CCNB1_HUMAN antibody
    • Cyclin B1 antibody
    • G2 mitotic specific cyclin B1 antibody
    • G2/mitotic-specific cyclin-B1 antibody
    see all

画像

  • All lanes : Anti-Cyclin B1 (phospho S147) antibody (ab60986) at 1/500 dilution

    Lane 1 : Extracts from Jurkat cells treated with UV light (15 mins).
    Lane 2 : Extracts from Jurkat cells treated with UV light (15 mins) and the immunising phosphopeptide.


    Predicted band size : 48 kDa
    Observed band size : >48 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemistry analysis of paraffin-embedded human placenta tissue using ab60986 at 1/50-1/100 dilution, with or without the immunising phosphopeptide.

参考文献

This product has been referenced in:
  • Holt JE  et al. Spatial regulation of APCCdh1-induced cyclin B1 degradation maintains G2 arrest in mouse oocytes. Development 137:1297-304 (2010). WB ; Mouse . Read more (PubMed: 20223764) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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