製品の概要

  • 製品名Anti-Cyclin B1 (phospho S147) antibody
    Cyclin B1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Cyclin B1 (phospho S147)
  • アプリケーション適用あり: ELISA, IHC-P, WBmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Synthetic peptide corresponding to Human Cyclin B1 (phospho S147). Immunogen range is aa 121-170.
    Database link: P14635

  • ポジティブ・コントロール
    • Human placenta tissue, Jurkat cell extracts treated with UV light.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab60986 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/10000.
IHC-P 1/50 - 1/100.
WB 1/500 - 1/1000. Detects a band of approximately >48 kDa (predicted molecular weight: 48 kDa).

ターゲット情報

  • 機能Essential for the control of the cell cycle at the G2/M (mitosis) transition.
  • 配列類似性Belongs to the cyclin family. Cyclin AB subfamily.
  • 発生段階Accumulates steadily during G2 and is abruptly destroyed at mitosis.
  • 翻訳後修飾Ubiquitinated by the SCF(NIPA) complex during interphase, leading to its destruction. Not ubiquitinated during G2/M phases.
  • 細胞内局在Cytoplasm. Nucleus. Cytoplasm > cytoskeleton > centrosome.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CCNB 1 antibody
    • CCNB antibody
    • ccnb1 antibody
    • CCNB1_HUMAN antibody
    • Cyclin B1 antibody
    • G2 mitotic specific cyclin B1 antibody
    • G2/mitotic-specific cyclin-B1 antibody
    see all

Anti-Cyclin B1 (phospho S147) antibody 画像

  • All lanes : Anti-Cyclin B1 (phospho S147) antibody (ab60986) at 1/500 dilution

    Lane 1 : Extracts from Jurkat cells treated with UV light (15 mins).
    Lane 2 : Extracts from Jurkat cells treated with UV light (15 mins) and the immunising phosphopeptide.


    Predicted band size : 48 kDa
    Observed band size : >48 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemistry analysis of paraffin-embedded human placenta tissue using ab60986 at 1/50-1/100 dilution, with or without the immunising phosphopeptide.

Anti-Cyclin B1 (phospho S147) antibody (ab60986) 使用論文

This product has been referenced in:
  • Holt JE  et al. Spatial regulation of APCCdh1-induced cyclin B1 degradation maintains G2 arrest in mouse oocytes. Development 137:1297-304 (2010). WB ; Mouse . Read more (PubMed: 20223764) »

See 1 Publication for this product

Product Wall

There are currently no Abreviews or Questions for ab60986.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"