製品の概要

  • 製品名
    Anti-Cyclin B1 (phospho S126) antibody
    Cyclin B1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Cyclin B1 (phospho S126)
  • 特異性
    ab55184 detects endogenous levels of Cyclin B1 only when phosphorylated at serine 126.
  • アプリケーション
    適用あり: ICC/IF, WB, ELISA, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Synthetic peptide corresponding to Human Cyclin B1 (phospho S126). Synthetic phosphopeptide derived from human Cyclin B1 around the phosphorylation site of serine 126 (T-A-SP-P-S).
    Database link: P14635

  • ポジティブ・コントロール
    • human lung carcinoma tissue, extracts from NIH/3T3 cells, treated with EGF.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab55184 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent concentration. PubMed: 19376971
WB 1/500 - 1/1000. Detects a band of approximately 65 kDa (predicted molecular weight: 48 kDa).
ELISA 1/5000.
IHC-P 1/50 - 1/100.

ターゲット情報

  • 機能
    Essential for the control of the cell cycle at the G2/M (mitosis) transition.
  • 配列類似性
    Belongs to the cyclin family. Cyclin AB subfamily.
  • 発生段階
    Accumulates steadily during G2 and is abruptly destroyed at mitosis.
  • 翻訳後修飾
    Ubiquitinated by the SCF(NIPA) complex during interphase, leading to its destruction. Not ubiquitinated during G2/M phases.
  • 細胞内局在
    Cytoplasm. Nucleus. Cytoplasm > cytoskeleton > centrosome.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CCNB 1 antibody
    • CCNB antibody
    • ccnb1 antibody
    • CCNB1_HUMAN antibody
    • Cyclin B1 antibody
    • G2 mitotic specific cyclin B1 antibody
    • G2/mitotic-specific cyclin-B1 antibody
    see all

画像

  • All lanes : Anti-Cyclin B1 (phospho S126) antibody (ab55184) at 1/500 dilution

    Lane 1 : extracts from NIH/3T3 cells treated with EGF (200ng/ml, 15mins).
    Lane 2 : extracts from NIH/3T3 cells treated with EGF (200ng/ml, 15mins); ab36621 pre-incubated with the immunising phosphopeptide.


    Predicted band size : 48 kDa
    Observed band size : 65 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemical analysis of Cyclin B1 (phospho S126) expression in paraffin embedded human lung carcinoma tissue using ab55184 at 1/50 dilution (left). Right hand panel represents a negative control where ab55184 was pre-incubated with the immunising (blocking) phosphopeptide.

参考文献

This product has been referenced in:
  • Müllers E  et al. Residual Cdk1/2 activity after DNA damage promotes senescence. Aging Cell 16:575-584 (2017). Read more (PubMed: 28345297) »
  • Álvarez-Fernández M  et al. Greatwall is essential to prevent mitotic collapse after nuclear envelope breakdown in mammals. Proc Natl Acad Sci U S A 110:17374-9 (2013). Mouse . Read more (PubMed: 24101512) »

See all 4 Publications for this product

レビューと Q&A

There are currently no Customer reviews or Questions for ab55184.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録