製品の概要

  • 製品名
    Anti-Cyclin B1 (phospho S126) antibody
    Cyclin B1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Cyclin B1 (phospho S126)
  • 特異性
    Minimal reactivity is expected with the non-phosphorylated form of the protein.
  • アプリケーション
    適用あり: WB, IPmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Hamster, Dog, Chimpanzee
  • 免疫原

    Synthetic peptide corresponding to Human Cyclin B1 aa 120-131 (phospho S126).
    Sequence: ILVDTASpPSPME
    Database link: P14635

  • 特記事項


    This antibody recognizes a Ser126 phosphorylation involved in an auto phosphorylation of the cdk -cyclin complex. Recognition of this phosphorylated site suggests the antibody is useful to detect the active form of the complex and may serve as a marker of mitosis.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: 0.01% Sodium Azide
    Constituents: 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 一次抗体 備考
    This antibody recognizes a Ser126 phosphorylation involved in an auto phosphorylation of the cdk -cyclin complex. Recognition of this phosphorylated site suggests the antibody is useful to detect the active form of the complex and may serve as a marker of mitosis.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab3488 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/100 - 1/1000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).
IP 1/100.

ターゲット情報

画像

  • All lanes : Anti-Cyclin B1 (phospho S126) antibody (ab3488) at 1/100 dilution

    Lane 1 : HeLa whole cell lysate
    Lane 2 : H23 whole cell lysate
    Lane 3 : Hepatocarcinoma (Hep3b) cell lysate
    Lane 4 : Glioblastoma (T98G) cell lysate
    Lane 5 : B cell lymphoblast (Daudi) cell lysate

    Lysates/proteins at 50 µg per lane.

    Secondary
    HRP-conjugated Goat anti-Rabbit IgG at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 48 kDa


    Exposure time : 20 seconds

参考文献

This product has been referenced in:

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