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RabMAb

Anti-Cyclin B1 抗体 [Y106] (ab32053)

製品の概要

  • 製品名
    Anti-Cyclin B1 antibody [Y106]
    Cyclin B1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [Y106] to Cyclin B1
  • 特異性
    This antibody is specific for Human cyclin B1. It does not cross-react with other cyclin family members.
  • アプリケーション
    適用あり: ICC/IF, WB, IHC-P, Flow Cyt, IPmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide corresponding to residues in the C-terminus of Human cyclin B1.

  • エピトープ
    ab32053 reacts with an epitope located in teh C terminal region of Cyclin B1.
  • ポジティブ・コントロール
    • Jurkat and Hela cell lysate.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    A trial size is available to purchase for this product.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Alternative versions available:

    Anti-Cyclin B1 antibody (HRP) [Y106] (ab193977)

    Anti-Cyclin B1 antibody (Phycoerythrin) [Y106] (ab210615)

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab32053 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/50.
WB 1/3000 - 1/20000. Predicted molecular weight: 58 kDa.
IHC-P Use at an assay dependent concentration.
Flow Cyt 1/20.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IP 1/100.

ターゲット情報

  • 機能
    Essential for the control of the cell cycle at the G2/M (mitosis) transition.
  • 配列類似性
    Belongs to the cyclin family. Cyclin AB subfamily.
  • 発生段階
    Accumulates steadily during G2 and is abruptly destroyed at mitosis.
  • 翻訳後修飾
    Ubiquitinated by the SCF(NIPA) complex during interphase, leading to its destruction. Not ubiquitinated during G2/M phases.
  • 細胞内局在
    Cytoplasm. Nucleus. Cytoplasm > cytoskeleton > centrosome.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CCNB 1 antibody
    • CCNB antibody
    • ccnb1 antibody
    • CCNB1_HUMAN antibody
    • Cyclin B1 antibody
    • G2 mitotic specific cyclin B1 antibody
    • G2/mitotic-specific cyclin-B1 antibody
    see all

画像



  • Predicted band size : 58 kDa

    Lane 1: Wild type HAP1 whole cell lysate (20 µg)
    Lane 2: CCNB1 knockout HAP1 whole cell lysate (20 µg)
    Lane 3: HeLa whole cell lysate (20 µg)
    Lanes 1 - 3: Merged signal (red and green). Green - ab32053 observed at 55 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab32053 was shown to specifically react with CCNB1 when CCNB1 knockout samples were used. Wild-type and CCNB1 knockout samples were subjected to SDS-PAGE. Ab32053 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 3000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • ab32053 at a 1:100 dilution staining Human cyclin B1 in human skin carcinoma, using Immunohistochemistry, Paraffin Embedded Tissue.
  • ab32053 staining Cyclin B1 in the U2OS cell line from human by ICC/IF ((Immunocytochemistry/immunofluorescence).Cells were fixed with formaldehyde,permeabilized with 1% Triton X-100 in PBS and blocked with 1% BSA for 1 hour at 37°C.Alexa Fluor® 594-conjugated goat anti-rabbit IgG polyclonal was used as the secondary antibody.

  • Overlay histogram showing Jurkat cells stained with ab32053 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab32053, 1/20 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) ( 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

  • All lanes : Anti-Cyclin B1 antibody [Y106] (ab32053) at 1/20000 dilution

    Lane 1 : Hela cell lysate.
    Lane 2 : Jurkat cell lysate.


    Predicted band size : 58 kDa

参考文献

This product has been referenced in:
  • Yang T  et al. Anti-tumor Efficiency of Lipid-coated Cisplatin Nanoparticles Co-loaded with MicroRNA-375. Theranostics 6:142-54 (2016). WB ; Human . Read more (PubMed: 26722380) »
  • Tseng YH  et al. Thyroid hormone suppresses expression of stathmin and associated tumor growth in hepatocellular carcinoma. Sci Rep 6:38756 (2016). Human . Read more (PubMed: 27934948) »

See all 26 Publications for this product

レビューと Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (U2OS)
Permeabilization
Yes - 0.1% TritonX-100
Specification
U2OS
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Formaldehyde
Username

Dr. Stephanie Hills

Verified customer

投稿 Apr 27 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
H2O2 as blocking agent for 10 minute(s) · Concentration: 3% · Temperature: 25°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: EDTA, pH 8.6, 20 min 100C
Sample
Human Tissue sections (tonsil)
Specification
tonsil
Permeabilization
No
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

投稿 Aug 06 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing (4-12%)
Sample
Human Cell lysate - whole cell (MCF7 cells)
Specification
MCF7 cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

投稿 Jul 11 2013

Thank you for contacting us.

I can confirm that the abs have IgG1 isotype.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa cells)
Loading amount
30 µg
Specification
HeLa cells
Treatment
double thymidine synchronization
Gel Running Conditions
Non-reduced Non-Denaturing (Native)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 27°C
Username

Abcam user community

Verified customer

投稿 May 14 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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