製品の概要

  • 製品名Anti-Cyclin B1 antibody
    Cyclin B1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Cyclin B1
  • 特異性Detects endogenous levels of total Cyclin B1 protein.
  • アプリケーション適用あり: ELISA, WB, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Synthetic peptide corresponding to Human Cyclin B1. Immunogen range is aa 91-140. Synthetic non-phosphopeptide derived from human Cyclin B1 around the phosphorylation site of serine 126
    Database link: P14635

  • ポジティブ・コントロール
    • Extracts from HeLa cells treated with serum.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab52187 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/20000.
WB 1/500 - 1/1000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).
IHC-P Use a concentration of 2 mg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

ターゲット情報

  • 機能Essential for the control of the cell cycle at the G2/M (mitosis) transition.
  • 配列類似性Belongs to the cyclin family. Cyclin AB subfamily.
  • 発生段階Accumulates steadily during G2 and is abruptly destroyed at mitosis.
  • 翻訳後修飾Ubiquitinated by the SCF(NIPA) complex during interphase, leading to its destruction. Not ubiquitinated during G2/M phases.
  • 細胞内局在Cytoplasm. Nucleus. Cytoplasm > cytoskeleton > centrosome.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CCNB 1 antibody
    • CCNB antibody
    • ccnb1 antibody
    • CCNB1_HUMAN antibody
    • Cyclin B1 antibody
    • G2 mitotic specific cyclin B1 antibody
    • G2/mitotic-specific cyclin-B1 antibody
    see all

Anti-Cyclin B1 antibody 画像

  • All lanes : Anti-Cyclin B1 antibody (ab52187) at 1/500 dilution

    Lane 1 : extracts from HeLa cells, treated with serum (10%, 15min)
    Lane 2 : extracts from HeLa cells, treated with serum (10%, 15min) with blocking peptide


    Predicted band size : 48 kDa
    Observed band size : 48 kDa
  • ab52187 (2µg/ml) staining Cyclin B1 in human placenta.
    Sections were stained using an automated system (DAKO Autostainer Plus ), at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers EDTA pH 9.0 . Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

Anti-Cyclin B1 antibody (ab52187) 使用論文

This product has been referenced in:
  • Park JE  et al. Direct quantification of polo-like kinase 1 activity in cells and tissues using a highly sensitive and specific ELISA assay. Proc Natl Acad Sci U S A 106:1725-1730 (2009). WB ; Mouse . Read more (PubMed: 19181852) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"