製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to Cyclin B1
  • アプリケーション
    適用あり: IHC-Pmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to Human Cyclin B1 (C terminal).
    Database link: P14635

  • ポジティブ・コントロール
    • Breast carcinoma.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab48574 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. ABC method.

ターゲット情報

Anti-Cyclin B1 antibody 画像

  • Ab48574 staining human Cyclin B1 in human breast carcinoma by immunohistochemistry using formalin fixed paraffin embedded tissue.
  • Ab48574 at 1/100 dilution staining Cyclin B1 in rat E19 embryonic brain sections by immunohistochemistry. Please see accompanying abreview for additional information.

    See Abreview

プロトコール

Anti-Cyclin B1 antibody (ab48574) 使用論文

This product has been referenced in:

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Cortical Brain Sections)
Specification
Cortical Brain Sections
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Sodium Citrate
Permeabilization
Yes - TBS with 1% triton
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Jun 11 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (E19 Embryonic Brain Sections)
Specification
E19 Embryonic Brain Sections
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Sodium Citrate
Permeabilization
Yes - PBS + 1% trition
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Mar 20 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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