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RabMAb

Anti-Cyclin A2 抗体 [E399] (ab32498)

製品の概要

  • 製品名
    Anti-Cyclin A2 antibody [E399]
    Cyclin A2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [E399] to Cyclin A2
  • 特異性
    ab32498 recognises Cyclin A2.
  • アプリケーション
    適用あり: WB, ICC/IF, IPmore details
    適用なし: Flow Cyt or IHC
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Cyclin A2 aa 100-200 (N terminal).

  • エピトープ
    ab32498 reacts with an epitope located in the N terminal region of Cyclin A2
  • ポジティブ・コントロール
    • WB: HeLa, K562 and Jurkat cell lysate. ICC/IF: HeLa cells.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    A trial size is available to purchase for this antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab32498 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/5000. Predicted molecular weight: 48 kDa.
ICC/IF 1/250 - 1/500.
IP 1/60.
  • 追加情報
    Is unsuitable for Flow Cyt or IHC.
  • ターゲット情報

    • 機能
      Essential for the control of the cell cycle at the G1/S (start) and the G2/M (mitosis) transitions.
    • 配列類似性
      Belongs to the cyclin family. Cyclin AB subfamily.
    • 発生段階
      Accumulates steadily during G2 and is abruptly destroyed at mitosis.
    • 細胞内局在
      Nucleus. Cytoplasm. Cytoplasmic when associated with SCAPER.
    • Information by UniProt
    • 参照データベース
    • 別名
      • CCN1 antibody
      • CCNA antibody
      • Ccna2 antibody
      • CCNA2_HUMAN antibody
      • Cyclin A2 antibody
      • Cyclin-A antibody
      • Cyclin-A2 antibody
      see all

    Anti-Cyclin A2 antibody [E399] 画像

    • All lanes : Anti-Cyclin A2 antibody [E399] (ab32498) at 1/1000 dilution

      Lane 1 : HeLa whole cell lysate
      Lane 2 : K562 whole cell lysate
      Lane 3 : Jurkat whole cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Peroxidase conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

      Predicted band size : 48 kDa
      Observed band size : 50 kDa (why is the actual band size different from the predicted?)


      Exposure time : 3 minutes

      Blocking and dilution buffer: 5% NFDM/TBST.

      The lower band is unidentified.

    • Immunofluorescent analysis of cyclin A expression in HeLa cells using 1/250 ab32498.

    Anti-Cyclin A2 antibody [E399] (ab32498) 使用論文

    This product has been referenced in:
    • Wei X  et al. Proteomics-based identification of the tumor suppressor role of aminoacylase 1 in hepatocellular carcinoma. Cancer Lett 351:117-25 (2014). WB ; Human . Read more (PubMed: 24846301) »
    • Yajima H  et al. The complexity of DNA double strand breaks is a critical factor enhancing end-resection. DNA Repair (Amst) 12:936-46 (2013). Read more (PubMed: 24041488) »

    See all 3 Publications for this product

    Product Wall

    Ich denke wirklich, dass Ihr Problem mit diesen Aks nicht auf den WB begrenzt ist , da mir die ICC Färbung nicht spezifisch erscheint. Ich würde die Färbung definitiv eher nuklear lokalisiert erwarten, und das ist sie einfach nicht.
    Wir verwenden ...

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    Ich habe Ihnen einen Blot mit dem ab32498 an diese Email gehängt: von einem Schmier keine Spur! Was mir allerdings aufgefallen ist, ist dass der Verdünnungsfaktor um 10 verkleinert wurde. Ich habe unser Datenblatt dementsprechend verändert. Falls ...

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    Thank you for your inquiry. The lab has not tested this antibody for its cross-reactivity with Cyclin A1. But when we BLASTed the immunogen sequence against Cyclin A1, we did not see any significant sequence similarity. Thus, we do not predict ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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