The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use at a concentration of 5 - 10 µg/ml for 30 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
IP: Use at 10µg/mg of lysate. Denatured verified.
WB: Use at a concentration of 5 - 10 µg/ml for 2hrs at RT. Predicted molecular weight: 49 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Essential for the control of the cell cycle at the G1/S (start) and the G2/M (mitosis) transitions.
Belongs to the cyclin family. Cyclin AB subfamily.
Accumulates steadily during G2 and is abruptly destroyed at mitosis.
Nucleus. Cytoplasm. Cytoplasmic when associated with SCAPER.
Lv XJ et al. RNA-binding motif protein 5 inhibits the proliferation of cigarette smoke-transformed BEAS-2B cells through cell cycle arrest and apoptosis. Oncol RepN/A:N/A (2016).
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