製品の概要

  • 製品名Anti-Cy5 antibody [CY5-15]
  • 製品の詳細
    Mouse monoclonal [CY5-15] to Cy5
  • 特異性ab52061 recognizes Cy5 and AlexaFluor647 labelled proteins, but not Cy3 labelled proteins.
  • アプリケーション適用あり: Dot blot, ELISA, IP, ICC, IHC-FoFr, Flow Cytmore details
  • 免疫原

    A mixture of proteins labelled with Cy5.

  • 特記事項


    Monoclonal antibodies to Cy5 are an important tool for Cy5 signal amplification in assays where biomolecules are labelled with Cy5.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 15mM Sodium Azide
    Constituents: 0.01M PBS, pH 7.4
  • Concentration information loading...
  • 特記事項(精製)Purified Immunoglobulin
  • 一次抗体 備考Monoclonal antibodies to Cy5 are an important tool for Cy5 signal amplification in assays where biomolecules are labelled with Cy5.
  • ポリ/モノモノクローナル
  • クローン名CY5-15
  • アイソタイプIgG1
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab52061 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Dot blot Use a concentration of 1 - 2 µg/ml.
ELISA Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.
IHC-FoFr 1/50.
Flow Cyt Use at an assay dependent concentration. PubMed: 24376610ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 関連性Cy3 and Cy5 are reactive watersoluble fluorescent dyes of the cyanine dye family used for labelling proteins, peptides, DNA, RNA, and other biomolecules. They are usually synthesized with reactive groups on either one or both of the nitrogen side chains so that they can be chemically cross-linked to nucleic acids or protein molecules. Thay are small, pH insensitive (between pH 3 and pH 10), soluble in aqueous solution and have tolerance to DMSO. They are more photostable than fluorescein, have high molar extinction coefficients and favorable quantum yields. They are used in many different biological assays, including DNA microarrays, protein microarrays, two-dimensional protein analysis, fluorescence resonance energy transfer, and immunocytochemistry. Cy3 is excited maximally at 550 nm and emits maximally at 570 nm, in the green part of the spectrum. Cy5 is excited maximally at 649 nm and emits maximally at 670 nm, in the red part of the spectrum.

Anti-Cy5 antibody [CY5-15] 画像

  • ab52061 staining Cy5 in rat brain tissue by Immunohistochemistry (Free Floating sections). Samples were incubated with the primary antibody at a 1/50 dilution for 20 hours at 20°C. A biotin-conjugated horse anti-mouse IgG, rat-adsorbed monoclonal was used as secondary antibody at a 1/200 dilution.

    See Abreview

Anti-Cy5 antibody [CY5-15] (ab52061) 使用論文

This product has been referenced in:
  • Cox JH  et al. Antibody-mediated targeting of the orai1 calcium channel inhibits T cell function. PLoS One 8:e82944 (2013). Flow Cyt . Read more (PubMed: 24376610) »

See 1 Publication for this product

Product Wall



Leider sehe ich ein paar Probleme bei diesem Protokoll:

ab52061 wurde nicht getestet ob er auch Cy5.5 erkennt. Das Immunogen war eine Mischung aus Cy5 konjugierten Proteinen.

Falls ab52061 Cy5.5 erkennen würde, hä...

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Unfortunately, ab52061 has not been tested against either Cy5.5 nor Cy7. Based on the dissimilarity in chemical structure of Cy5.5 and Cy7 vs Cy5, it seems unlikely that ab52061 will recognize either Cy5.5 or Cy7.

Thank you for contacting us.

Unfortunately we do not have any images of product ab52061 and ab52060 with Alexa 647. Alexa 647 is based on the structure of Cy5 and therefore is structurally very similar. As our data sheet states this antibody...

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Thank you for your inquiry.

Unfortunately I am sorry to confirm we have no data or experimental information to verify whether ab52061 Cy5 antibody [CY5-15]cross reacts with Cy5.5. The Cy5 and Cy5.5 structures are quite similar and so the pos...

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Thank you for contacting us.

Unfortunately we have no data or experimental information to verify whether product ab52061 (anti-Cy5) cross reacts with AF-680.

Please do not hesitate to contact us if you need any more advice or info...

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Thank you for your inquiry.

Unfortunately we have no information on whether this antibody cross-reacts with Cy5.5. However looking at a comparison of the molecules that I have attached I believe that it is probably highly unlikely that it wil...

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Thank you and your customer for your inquiry.

I am happy to confirm that ab52061 will only bind to Cy5 and not to the Rhodamine related Tamra or to biotin.

This is tested and guaranteed by our Abpromise.

I wish your custom...

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Application Immunohistochemistry free floating
Sample Rat Tissue sections (brain)
Specification brain
Username

Mrs. Gitte Toft

Verified customer

投稿 Nov 04 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"